TY - JOUR
T1 - Effect of Phosphate-based Glass Porous Microspheres (P30) Loaded with Extracellular Vesicle on Osteoblast Behaviour
T2 - In Vitro Study
AU - Cahayadi, Sigit Daru
AU - Nuzulia, Nur Aisyah
AU - Boediono, Arief
AU - Ahmed, Ifty
AU - Nurhayati, Retno Wahyu
AU - Sari, Yessie Widya
AU - Juliandi, Berry
N1 - Publisher Copyright:
© 2025@ author(s).
PY - 2025/7
Y1 - 2025/7
N2 - Bioactive materials, particularly phosphate-based glasses (PBG), hold great promise in bone repair due to their controllable degradation rates and bioactivity. This study evaluated PBG porous microspheres (P30) loaded with extracellular vesicles (EVs) for bone tissue engineering, focusing on osteogenesis, EV uptake, and cell invasion. P30 concentrations (5P30, 10P30, 50P30, 100P30, 500P30) were tested for their effects on calcification, EV uptake, and cell migration. Results showed that 100P30 exhibited optimal conditions for osteogenesis and EV delivery, with the highest calcification areas at both Day 7 and Day 14 and the most efficient EV internalization. Meanwhile, 500P30 demonstrated the highest cell migration, supporting pre-osteoblastic migration at this concentration. These findings indicate that 100P30 is ideal for mineralization and EV uptake, while 500P30 enhances cell invasion. This study highlights P30's versatility as a biomaterial for bone regeneration, with specific concentrations tailored to different regenerative goals. These results underscore the potential of P30 microspheres loaded with EVs as an effective strategy for promoting bone repair and regeneration.
AB - Bioactive materials, particularly phosphate-based glasses (PBG), hold great promise in bone repair due to their controllable degradation rates and bioactivity. This study evaluated PBG porous microspheres (P30) loaded with extracellular vesicles (EVs) for bone tissue engineering, focusing on osteogenesis, EV uptake, and cell invasion. P30 concentrations (5P30, 10P30, 50P30, 100P30, 500P30) were tested for their effects on calcification, EV uptake, and cell migration. Results showed that 100P30 exhibited optimal conditions for osteogenesis and EV delivery, with the highest calcification areas at both Day 7 and Day 14 and the most efficient EV internalization. Meanwhile, 500P30 demonstrated the highest cell migration, supporting pre-osteoblastic migration at this concentration. These findings indicate that 100P30 is ideal for mineralization and EV uptake, while 500P30 enhances cell invasion. This study highlights P30's versatility as a biomaterial for bone regeneration, with specific concentrations tailored to different regenerative goals. These results underscore the potential of P30 microspheres loaded with EVs as an effective strategy for promoting bone repair and regeneration.
KW - bioactive potential
KW - bone regeneration
KW - osteogenesis
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=105003950605&partnerID=8YFLogxK
U2 - 10.4308/hjb.32.4.840-849
DO - 10.4308/hjb.32.4.840-849
M3 - Article
AN - SCOPUS:105003950605
SN - 1978-3019
VL - 32
SP - 840
EP - 849
JO - HAYATI Journal of Biosciences
JF - HAYATI Journal of Biosciences
IS - 4
ER -