Effect of nanocurcumin on cisplatin-induced acute kidney injury: A focus on NRF2 and keap

Bashar Adi Wahyu Pandhita; Vivian Soetikno; Melva Louisa

doi:10.22159/ijap.2018.v10s1.32

Effect of nanocurcumin on cisplatin-induced acute kidney injury: A focus on NRF2 and keap

Bashar Adi Wahyu Pandhita, Vivian Soetikno, Melva Louisa

Department of Pharmacology and Therapeutic Pre Clinic

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1. Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1. Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop, which upregulated transcription of Keap1. Conclusion: NC increased Keap1 levels, but not significantly.

Original language	English
Pages (from-to)	152-154
Number of pages	3
Journal	International Journal of Applied Pharmaceutics
Volume	10
Issue number	Special Issue 1
DOIs	https://doi.org/10.22159/ijap.2018.v10s1.32
Publication status	Published - 1 Dec 2018

Keywords

Cisplatin
Keap1
Nanocurcumin
Nephrotoxicity
Nrf2

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10.22159/ijap.2018.v10s1.32

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title = "Effect of nanocurcumin on cisplatin-induced acute kidney injury: A focus on NRF2 and keap",

abstract = "Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1. Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1. Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop, which upregulated transcription of Keap1. Conclusion: NC increased Keap1 levels, but not significantly.",

keywords = "Cisplatin, Keap1, Nanocurcumin, Nephrotoxicity, Nrf2",

author = "Pandhita, {Bashar Adi Wahyu} and Vivian Soetikno and Melva Louisa",

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AU - Pandhita, Bashar Adi Wahyu

AU - Soetikno, Vivian

AU - Louisa, Melva

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1. Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1. Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop, which upregulated transcription of Keap1. Conclusion: NC increased Keap1 levels, but not significantly.

AB - Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1. Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1. Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop, which upregulated transcription of Keap1. Conclusion: NC increased Keap1 levels, but not significantly.

KW - Cisplatin

KW - Keap1

KW - Nanocurcumin

KW - Nephrotoxicity

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Effect of nanocurcumin on cisplatin-induced acute kidney injury: A focus on NRF2 and keap

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Keywords

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