Objective: This study aimed to investigate the effect of nanocurcumin (NC) against cisplatin-induced acute kidney injury, focusing on Nrf2 and Keap1. Methods: Male Sprague-Dawley rats (n=23) were divided into five groups (Control, CP, CP+Cur, CP+50 NC, and CP+100 NC) and sacrificed 7 days after treatment. Whole kidneys were collected for reverse transcription polymerase chain reaction (RT-PCR) analysis of Nrf2 and Keap1. Results: There were no statistically significant differences in the RT-PCR results among the groups (p>0.05). However, Keap1 expression was upregulated in rats treated with 100 mg of NC, which may have been caused by increased Nrf2 activation and activation of a negative feedback loop, which upregulated transcription of Keap1. Conclusion: NC increased Keap1 levels, but not significantly.