Lately, artificial surfactant is more often used than natural surfactant for petroleum industry. The main advantages of using natural surfactant is because the continuous availability. Single Cell Oil, which utillizes one-cell microorganism, can be a solution, such as Aspergillus oryzae, to produce AA, DHA & EPA. A. oryzae was cultivated on Potato Dextrose Agar (PDA), Czapek Dox Agar (CDA) and Malt Extract Agar (MEA), and then the incubation times are 2,4,5,6 and 7 days in optimal medium. Lipids were extracted using ethanol and n-hexane. The characterization of lipid was done by gas chromatography (GC) method. The most optimal medium is CDA with a lipid concetration of 0,18 g. The best incubation time on CDA medium was 5 days with 0,216 g lipid content containing 0,123 g unsaturated fatty acid, 0,0613 g PUFA and 0,0615 g MUFA. The unsaturated fatty acid composition produced on the 5th day was 29.2% oleate; 29.1% linoleate and ± 0.046% EPA.
|Journal||IOP Conference Series: Earth and Environmental Science|
|Publication status||Published - 25 Jan 2018|
|Event||2nd International Tropical Renewable Energy Conference, i-TREC 2017 - Bali, Indonesia|
Duration: 3 Oct 2017 → 4 Oct 2017