TY - JOUR
T1 - Effect of medium and incubation time on production of AA, DHA and EPA from Aspergillus oryzae by solid state fermentation
AU - Pinasthika, N. P.
AU - Arbianti, Rita
AU - Utami, Tania Surya
AU - Hermansyah, Heri
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/1/25
Y1 - 2018/1/25
N2 - Lately, artificial surfactant is more often used than natural surfactant for petroleum industry. The main advantages of using natural surfactant is because the continuous availability. Single Cell Oil, which utillizes one-cell microorganism, can be a solution, such as Aspergillus oryzae, to produce AA, DHA & EPA. A. oryzae was cultivated on Potato Dextrose Agar (PDA), Czapek Dox Agar (CDA) and Malt Extract Agar (MEA), and then the incubation times are 2,4,5,6 and 7 days in optimal medium. Lipids were extracted using ethanol and n-hexane. The characterization of lipid was done by gas chromatography (GC) method. The most optimal medium is CDA with a lipid concetration of 0,18 g. The best incubation time on CDA medium was 5 days with 0,216 g lipid content containing 0,123 g unsaturated fatty acid, 0,0613 g PUFA and 0,0615 g MUFA. The unsaturated fatty acid composition produced on the 5th day was 29.2% oleate; 29.1% linoleate and ± 0.046% EPA.
AB - Lately, artificial surfactant is more often used than natural surfactant for petroleum industry. The main advantages of using natural surfactant is because the continuous availability. Single Cell Oil, which utillizes one-cell microorganism, can be a solution, such as Aspergillus oryzae, to produce AA, DHA & EPA. A. oryzae was cultivated on Potato Dextrose Agar (PDA), Czapek Dox Agar (CDA) and Malt Extract Agar (MEA), and then the incubation times are 2,4,5,6 and 7 days in optimal medium. Lipids were extracted using ethanol and n-hexane. The characterization of lipid was done by gas chromatography (GC) method. The most optimal medium is CDA with a lipid concetration of 0,18 g. The best incubation time on CDA medium was 5 days with 0,216 g lipid content containing 0,123 g unsaturated fatty acid, 0,0613 g PUFA and 0,0615 g MUFA. The unsaturated fatty acid composition produced on the 5th day was 29.2% oleate; 29.1% linoleate and ± 0.046% EPA.
UR - http://www.scopus.com/inward/record.url?scp=85041688984&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/105/1/012104
DO - 10.1088/1755-1315/105/1/012104
M3 - Conference article
AN - SCOPUS:85041688984
SN - 1755-1307
VL - 105
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012104
T2 - 2nd International Tropical Renewable Energy Conference, i-TREC 2017
Y2 - 3 October 2017 through 4 October 2017
ER -