Effect of medium and incubation time on production of AA, DHA and EPA from Aspergillus oryzae by solid state fermentation

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Abstract

Lately, artificial surfactant is more often used than natural surfactant for petroleum industry. The main advantages of using natural surfactant is because the continuous availability. Single Cell Oil, which utillizes one-cell microorganism, can be a solution, such as Aspergillus oryzae, to produce AA, DHA & EPA. A. oryzae was cultivated on Potato Dextrose Agar (PDA), Czapek Dox Agar (CDA) and Malt Extract Agar (MEA), and then the incubation times are 2,4,5,6 and 7 days in optimal medium. Lipids were extracted using ethanol and n-hexane. The characterization of lipid was done by gas chromatography (GC) method. The most optimal medium is CDA with a lipid concetration of 0,18 g. The best incubation time on CDA medium was 5 days with 0,216 g lipid content containing 0,123 g unsaturated fatty acid, 0,0613 g PUFA and 0,0615 g MUFA. The unsaturated fatty acid composition produced on the 5th day was 29.2% oleate; 29.1% linoleate and ± 0.046% EPA.

Original languageEnglish
Article number012104
JournalIOP Conference Series: Earth and Environmental Science
Volume105
Issue number1
DOIs
Publication statusPublished - 25 Jan 2018
Event2nd International Tropical Renewable Energy Conference, i-TREC 2017 - Bali, Indonesia
Duration: 3 Oct 20174 Oct 2017

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