TY - JOUR
T1 - Effect of hypoxia-inducible factor-1α induction by CoCL2 on breast cancer cells survival
T2 - Influence of cytochrome-c and survivin
AU - Paramita, Reni
AU - Sadikin, Mohamad
AU - Sutandyo, Noorwati
AU - Wanandi, Septelia Inawati
N1 - Publisher Copyright:
© 2014 Authors.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation. Methods: Breast cancer cell lines T47D were induced by CoCl2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities. Results: HIF-1α induction by CoCl2 causes the increase of protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability. Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.
AB - Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation. Methods: Breast cancer cell lines T47D were induced by CoCl2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities. Results: HIF-1α induction by CoCl2 causes the increase of protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability. Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.
KW - Apoptosis
KW - Cell viability
KW - Cytochrome-c
KW - Hypoxia-inducible factor-1α
KW - Survivin
UR - http://www.scopus.com/inward/record.url?scp=85009069333&partnerID=8YFLogxK
U2 - 10.13181/mji.v23i3.933
DO - 10.13181/mji.v23i3.933
M3 - Article
AN - SCOPUS:85009069333
SN - 0853-1773
VL - 23
SP - 139
EP - 146
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
IS - 3
ER -