TY - JOUR
T1 - Effect of elevated temperature on SARS-CoV-2 viability
AU - Harapan, Harapan
AU - Johar, Edison
AU - Maroef, Chairin Nisa
AU - Sriyani, Ida Yus
AU - Iqhrammullah, Muhammad
AU - Kusuma, Hendrix Indra
AU - Syukri, Maimun
AU - Razali, Razali
AU - Hamdani, Hamdani
AU - Kurniawan, Rudi
AU - Irwansyah, Irwansyah
AU - Sofyan, Sarwo Edhy
AU - Myint, Khin Saw
AU - Mahlia, T. M.Indra
AU - Rizal, Samsul
N1 - Funding Information:
This research was funded by Lembaga Pengelola Dana Pendidikan (LPDP), managed by Indonesian Science Fund (ISF) (Grant No RISPRO/KI/B1/TKL/5/15448/2020).
Publisher Copyright:
Copyright: © 2022 Harapan H et al.
PY - 2022
Y1 - 2022
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 13 PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 13 PFU/mL to 10 9 PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 13 PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 13 PFU/mL to 10 9 PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.
KW - COVID-19
KW - Isolation chamber
KW - SARS-CoV-2
KW - Temperature
KW - Transmission
UR - http://www.scopus.com/inward/record.url?scp=85153848326&partnerID=8YFLogxK
U2 - 10.12688/f1000research.110305.1
DO - 10.12688/f1000research.110305.1
M3 - Article
AN - SCOPUS:85153848326
SN - 2046-1402
VL - 11
JO - F1000Research
JF - F1000Research
M1 - 403
ER -