Effect of elevated temperature on SARS-CoV-2 viability

Harapan Harapan, Edison Johar, Chairin Nisa Maroef, Ida Yus Sriyani, Muhammad Iqhrammullah, Hendrix Indra Kusuma, Maimun Syukri, Razali Razali, Hamdani Hamdani, Rudi Kurniawan, Irwansyah Irwansyah, Sarwo Edhy Sofyan, Khin Saw Myint, T. M.Indra Mahlia, Samsul Rizal

Research output: Contribution to journalArticlepeer-review


Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 13 PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 13 PFU/mL to 10 9 PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.

Original languageEnglish
Article number403
Publication statusPublished - 2022


  • COVID-19
  • Isolation chamber
  • SARS-CoV-2
  • Temperature
  • Transmission


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