TY - JOUR
T1 - Effect of egg yolk on the spermatozoa quality of the botia chromobotia macracanthus (Bleeker, 1852) (cyprinidae) after short-term cryopreservation
AU - Abinawanto,
AU - Wulandari, Rinda
AU - Muchlisin, Zainal A.
N1 - Funding Information:
This study was supported by a research grant from Universitas Indonesia with Contract No. 609/UN2.R3.1/HKP.05.00/2017. Special thanks to Taryana and Asri Martini for their kind assistance.
Publisher Copyright:
© 2018, BIOFLUX SRL. All rights reserved.
PY - 2018/12/30
Y1 - 2018/12/30
N2 - The egg yolk effect combined with 10% of methanol to preserve botia, Chromobotia macracanthus spermatozoa for 24 hours in liquid nitrogen has been studied. The concentration of egg yolk used in this study were, 0%, 5%, 7%, 9%, 11%, 13%, 15%, and 17%, respectively. As the positive control, the synthetic cryoprotectant carboxy methyl cellulose (CMC) was used. The sperms were diluted with the combination of 10% methanol, glucose extender and egg yolk (1 part of sample + 4 parts of solvent) and were equilibrated for 2 hours at 4°C before freezed for 24 hours in liquid nitrogen. After freezed, sample (sperms) were thawed for 13 sec at 40°C. Spermatozoa viability, motility, and abnormality, were then observed. Ten percent of methanol combined with 15% of egg yolk could preserve spermatozoa which maintain either sperm motility (96.43±1.49%) or sperm viability (84.25±1.26%) and reduced the abnormality up to 11.50±1.29%, respectively. Besides, the combination of 15% of egg yolk and 10% of methanol showed the highest fertility rate (50.64±4.37%).
AB - The egg yolk effect combined with 10% of methanol to preserve botia, Chromobotia macracanthus spermatozoa for 24 hours in liquid nitrogen has been studied. The concentration of egg yolk used in this study were, 0%, 5%, 7%, 9%, 11%, 13%, 15%, and 17%, respectively. As the positive control, the synthetic cryoprotectant carboxy methyl cellulose (CMC) was used. The sperms were diluted with the combination of 10% methanol, glucose extender and egg yolk (1 part of sample + 4 parts of solvent) and were equilibrated for 2 hours at 4°C before freezed for 24 hours in liquid nitrogen. After freezed, sample (sperms) were thawed for 13 sec at 40°C. Spermatozoa viability, motility, and abnormality, were then observed. Ten percent of methanol combined with 15% of egg yolk could preserve spermatozoa which maintain either sperm motility (96.43±1.49%) or sperm viability (84.25±1.26%) and reduced the abnormality up to 11.50±1.29%, respectively. Besides, the combination of 15% of egg yolk and 10% of methanol showed the highest fertility rate (50.64±4.37%).
KW - Chromobotia macracanthus
KW - Egg yolk
KW - Fertility rate
KW - Spermatozoa preservation
UR - http://www.scopus.com/inward/record.url?scp=85060229468&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85060229468
VL - 11
SP - 1737
EP - 1744
JO - AACL Bioflux
JF - AACL Bioflux
SN - 1844-8143
IS - 6
ER -