Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short-term cryopreservation

Zainal A. Muchlisin; Putri I. Sarah; Dhea F. Aldila; Kartini Eriani; Iwan Hasri; Agung S. Batubara; Firman M. Nur; Mustaqim Mustaqim; Cut Ruhul Muthmainnah; Abinawanto Abinawanto; Martin Wilkes

doi:10.1111/are.14516

Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short-term cryopreservation

Zainal A. Muchlisin, Putri I. Sarah, Dhea F. Aldila, Kartini Eriani, Iwan Hasri, Agung S. Batubara, Firman M. Nur, Mustaqim Mustaqim, Cut Ruhul Muthmainnah, Abinawanto Abinawanto, Martin Wilkes

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non-permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non-permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p <.05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk.

Original language	English
Pages (from-to)	1700-1705
Number of pages	6
Journal	Aquaculture Research
Volume	51
Issue number	4
DOIs	https://doi.org/10.1111/are.14516
Publication status	Published - 1 Apr 2020

Keywords

artificial breeding
endemic fish
extracellular CPA
permeating cryoprotectant

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10.1111/are.14516

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Muchlisin, Z. A., Sarah, P. I., Aldila, D. F., Eriani, K., Hasri, I., Batubara, A. S., Nur, F. M., Mustaqim, M., Muthmainnah, C. R., Abinawanto, A., & Wilkes, M. (2020). Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short-term cryopreservation. Aquaculture Research, 51(4), 1700-1705. https://doi.org/10.1111/are.14516

Muchlisin, Zainal A. ; Sarah, Putri I. ; Aldila, Dhea F. ; Eriani, Kartini ; Hasri, Iwan ; Batubara, Agung S. ; Nur, Firman M. ; Mustaqim, Mustaqim ; Muthmainnah, Cut Ruhul ; Abinawanto, Abinawanto ; Wilkes, Martin. / Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces : Cyprinidae) eggs after short-term cryopreservation. In: Aquaculture Research. 2020 ; Vol. 51, No. 4. pp. 1700-1705.

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title = "Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short-term cryopreservation",

abstract = "Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non-permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non-permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p <.05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk.",

keywords = "artificial breeding, endemic fish, extracellular CPA, permeating cryoprotectant",

author = "Muchlisin, {Zainal A.} and Sarah, {Putri I.} and Aldila, {Dhea F.} and Kartini Eriani and Iwan Hasri and Batubara, {Agung S.} and Nur, {Firman M.} and Mustaqim Mustaqim and Muthmainnah, {Cut Ruhul} and Abinawanto Abinawanto and Martin Wilkes",

note = "Funding Information: This study was supported by Kemenristek Dikti Republic of Indonesia through Applied Research Scheme 2018 Contract No. 91/UN11.2/PP/SP3/2018. Therefore, the authors thank Kemenristek Dikti Republic of Indonesia for supporting this study by providing the research grant for the first author. Publisher Copyright: {\textcopyright} 2020 John Wiley & Sons Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = apr,

day = "1",

doi = "10.1111/are.14516",

language = "English",

volume = "51",

pages = "1700--1705",

journal = "Aquaculture Research",

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Muchlisin, ZA, Sarah, PI, Aldila, DF, Eriani, K, Hasri, I, Batubara, AS, Nur, FM, Mustaqim, M, Muthmainnah, CR, Abinawanto, A & Wilkes, M 2020, 'Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short-term cryopreservation', Aquaculture Research, vol. 51, no. 4, pp. 1700-1705. https://doi.org/10.1111/are.14516

Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces : Cyprinidae) eggs after short-term cryopreservation. / Muchlisin, Zainal A.; Sarah, Putri I.; Aldila, Dhea F.; Eriani, Kartini; Hasri, Iwan; Batubara, Agung S.; Nur, Firman M.; Mustaqim, Mustaqim; Muthmainnah, Cut Ruhul; Abinawanto, Abinawanto; Wilkes, Martin.

In: Aquaculture Research, Vol. 51, No. 4, 01.04.2020, p. 1700-1705.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces

T2 - Cyprinidae) eggs after short-term cryopreservation

AU - Muchlisin, Zainal A.

AU - Sarah, Putri I.

AU - Aldila, Dhea F.

AU - Eriani, Kartini

AU - Hasri, Iwan

AU - Batubara, Agung S.

AU - Nur, Firman M.

AU - Mustaqim, Mustaqim

AU - Muthmainnah, Cut Ruhul

AU - Abinawanto, Abinawanto

AU - Wilkes, Martin

N1 - Funding Information: This study was supported by Kemenristek Dikti Republic of Indonesia through Applied Research Scheme 2018 Contract No. 91/UN11.2/PP/SP3/2018. Therefore, the authors thank Kemenristek Dikti Republic of Indonesia for supporting this study by providing the research grant for the first author. Publisher Copyright: © 2020 John Wiley & Sons Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non-permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non-permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p <.05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk.

AB - Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non-permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non-permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p <.05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk.

KW - artificial breeding

KW - endemic fish

KW - extracellular CPA

KW - permeating cryoprotectant

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U2 - 10.1111/are.14516

DO - 10.1111/are.14516

M3 - Article

AN - SCOPUS:85078798293

VL - 51

SP - 1700

EP - 1705

JO - Aquaculture Research

JF - Aquaculture Research

SN - 1355-557X

IS - 4

ER -

Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short-term cryopreservation

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Keywords

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