Effect of date palm (Phoenix dactylifera l.) on spermatozoa viability of kancra fish (Tor soro Valenciennes 1842) 48 hours post cryopreservation

Kancra is an endemic fish used in traditional functions in different parts of Indonesia. However, the population of the fish is decreasing due to environmental damage and excessive fishing. Cryopreservation of sperm of kancra fish is one of the most effective approaches to solving this problem. One of the success factors of cryopreservation is cryoprotectant. This study aims to evaluate the date palm juice as a natural cryoprotectant on the viability percentage of kancra fish after 48 hours of post cryopreservation. The sperm was collected by stripping and was diluted in the soluble liquid (fish ringer solution+methanol 10%+ date palm juice). The date palm juice concentration used were 0%, 5%, 10%, 15%, 20% and 25%. The sperm was equilibrated for 10 minutes at the 5°C temperature before it was frozen for 48 hours at the-10 °C. The thawing was done for 1 minute at 40 °C. Data were analyzed using ANOVA and followed by Tukey test. The result showed there was an effect of date palm juice (p<0.05) on the viability percentage of sperm post cryopreservation. The 10% concentration of date palm juice was optimum (p<0.05) with the percentage of viability 80.75 ± 1.19%.