Heat pipes have been widely used as one of the alternative methods to absorb more heat in the cooling systems of electronic devices. One of the ways to improve the thermal performance of heat pipes is to change the fluid transport properties and flow features of working fluids using anofluids. The purpose of this research was to investigate the effect of Al2O3-water nanofluids concentration and fluid loading to the thermal resistance between evaporator and adiabatic section of copper straight sintered copper powder wick heat pipe. In this research, sintered powder wick heat pipes were manufactured and tested to determine the thermal resistance of the sintered powder wick heat pipes which charged with water and Al2O3-water nanofluids. The concentrations of the nanoparticles were varied from 1%, 3% and 5% of the volume of the base fluid. The result shows that Al2O3-water nanofluids have the ability to reduce the temperature at the evaporator section and the thermal resistance of heat pipe. The increase in nanofluids concentration could give significant effect to reduce the thermal resistance of heat pipes. The amount of working fluid charged into the heat pipes also gives an effect in heat pipes thermal resistance, where the thermal resistance was lower when the heat pipe was charged with 60% of its volume. The formation of coating layer at sintered powder wick also can fixed the wick porosity and cause roughness on the surface of granular pore which the increased of capillary could give the effect for enhancement of heat pipe performance.