Effect of chitosan's amino group in adsorption-crosslinking immobilization of lipase enzyme on resin to catalyze biodiesel synthesis

Gandhi Alamsyah, Vania Anisya Albels, Muhamad Sahlan, Heri Hermansyah

Research output: Contribution to journalConference articlepeer-review

8 Citations (Scopus)

Abstract

Lipase as biocatalyst is used in biodiesel production but its price is becoming serious problem. Immobilization could improve the ability of enzyme. Immobilization method which gives higher activity and stability is adsorption-crosslinking method. The addition of amino group on supports has proven to stabilize the enzyme. Thus, this research focused on the improvement of lipase immobilization performance by the addition of chitosan which contains amino group. The highest unit activity (24.69 U/g resin) is reached by immobilized lipase on anion-exchange macroporous resin with addition of chitosan on resin directly. This enzyme produces biodiesel with yield of 50.79%.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalEnergy Procedia
Volume136
DOIs
Publication statusPublished - 1 Jan 2017
Event4th International Conference on Energy and Environment Research, ICEER 2017 - Porto, Portugal
Duration: 17 Jul 201720 Jul 2017

Keywords

  • Adsorption-crosslinking
  • chitosan
  • immobilization
  • lipase
  • resin

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