Chitosan is a natural polysaccharide that is nontoxic, easily chemically modified, and inhibits microbial activity. Chitosan is widely used for various purposes, one of which is as a membrane material in microencapsulation technology. This study aimed to get the optimum chitosan loading on the synthesis of CuO/TiO2 and encapsulated essential oils to improve antibacterial performance. CuO dopant was deposited on TiO2 by PAD (Photo Assisted Deposition) method to improve the photocatalytic performance. CuO/TiO2 was characterized by UV-Vis DRS. CuO/TiO2 and encapsulated essential oils were coated on the fabric. The bacteria disinfection ability on fabrics was evaluated using Escherichia coli bacteria. Then, the UV-Vis DRS results showed that the presence of CuO reduced the band-gap energy of TiO2. The antibacterial test results showed that modified fabric by CuO/TiO2 and essential oil encapsulated with 1,5% chitosan was the optimum sample, with Escherichia coli bacteria disinfection reached 50% in 120 minutes irradiation.