Effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile ≥30 kDa

B. D. Baskoro, R. A. Nugraha, Ria Puspitawati, S. Redjeki

Research output: Contribution to journalConference articlepeer-review

3 Citations (Scopus)

Abstract

There are no established standard operational procedures of centrifugation for protein separation. Centrifugation at 10,000 g separates ≥30 kDa salivary proteins. The aim of this study is to determine the effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile and the frequency of ≥30 kDa salivary proteins. Post-centrifuged salivary supernatant was analyzed using SDS-PAGE. An increased centrifugation speed resulted in the decreased frequency of ≥30 kDa salivary protein emergence. Each centrifugation speed resulted in a different protein profile. Centrifugation at 7,000 g, 8,000 g, and 9,000 g influences the frequency of salivary protein emergence and protein profiles ≥30 kDa.

Original languageEnglish
Article number012013
JournalJournal of Physics: Conference Series
Volume884
Issue number1
DOIs
Publication statusPublished - 30 Aug 2017
Event1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017 - Depok, West Java, Indonesia
Duration: 15 Jul 201716 Jul 2017

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