TY - JOUR
T1 - Effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile ≥30 kDa
AU - Baskoro, B. D.
AU - Nugraha, R. A.
AU - Puspitawati, Ria
AU - Redjeki, S.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2017/8/30
Y1 - 2017/8/30
N2 - There are no established standard operational procedures of centrifugation for protein separation. Centrifugation at 10,000 g separates ≥30 kDa salivary proteins. The aim of this study is to determine the effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile and the frequency of ≥30 kDa salivary proteins. Post-centrifuged salivary supernatant was analyzed using SDS-PAGE. An increased centrifugation speed resulted in the decreased frequency of ≥30 kDa salivary protein emergence. Each centrifugation speed resulted in a different protein profile. Centrifugation at 7,000 g, 8,000 g, and 9,000 g influences the frequency of salivary protein emergence and protein profiles ≥30 kDa.
AB - There are no established standard operational procedures of centrifugation for protein separation. Centrifugation at 10,000 g separates ≥30 kDa salivary proteins. The aim of this study is to determine the effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile and the frequency of ≥30 kDa salivary proteins. Post-centrifuged salivary supernatant was analyzed using SDS-PAGE. An increased centrifugation speed resulted in the decreased frequency of ≥30 kDa salivary protein emergence. Each centrifugation speed resulted in a different protein profile. Centrifugation at 7,000 g, 8,000 g, and 9,000 g influences the frequency of salivary protein emergence and protein profiles ≥30 kDa.
UR - http://www.scopus.com/inward/record.url?scp=85029586746&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/884/1/012013
DO - 10.1088/1742-6596/884/1/012013
M3 - Conference article
AN - SCOPUS:85029586746
SN - 1742-6588
VL - 884
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012013
T2 - 1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017
Y2 - 15 July 2017 through 16 July 2017
ER -