Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells

Yalista Fatia Nadia, Resda Akhra Syahrani, Sekar Arumsari, Mohamad Sadikin, Septelia Inawati Wanandi

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalIndonesian Biomedical Journal
Volume13
Issue number4
DOIs
Publication statusPublished - 2021

Keywords

  • breast cancer
  • cancer stem cell
  • OCT4
  • proliferation
  • stemness

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