Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells

Yalista Fatia Nadia; Resda Akhra Syahrani; Sekar Arumsari; Mohamad Sadikin; Septelia Inawati Wanandi

doi:10.18585/INABJ.V13I4.1674

Effect of Cell Culture Medium on the Proliferation and Stemness of CD24^-/CD44⁺ Human Breast Cancer Stem Cells

Yalista Fatia Nadia, Resda Akhra Syahrani, Sekar Arumsari, Mohamad Sadikin, Septelia Inawati Wanandi

Department of Biochemistry and Molecular Biology Pre Clinic

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24^-/CD44⁺ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES.

Original language	English
Pages (from-to)	355-363
Number of pages	9
Journal	Indonesian Biomedical Journal
Volume	13
Issue number	4
DOIs	https://doi.org/10.18585/INABJ.V13I4.1674
Publication status	Published - 2021

Keywords

breast cancer
cancer stem cell
OCT4
proliferation
stemness

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.18585/INABJ.V13I4.1674

Cite this

@article{410fe0aa10da439585d69ec3c47e0d97,

title = "Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells",

abstract = "BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES.",

keywords = "breast cancer, cancer stem cell, OCT4, proliferation, stemness",

author = "Nadia, {Yalista Fatia} and Syahrani, {Resda Akhra} and Sekar Arumsari and Mohamad Sadikin and Wanandi, {Septelia Inawati}",

note = "Funding Information: The authors are thankful for the Research Grant (Penelitian Dasar Unggulan Perguruan Tinggi/PDUPT/ PDUPT NKB-2777/UN2.RST/HKP.05.00/2020) from Directorate General of Research and Development Strengthening, Ministry of Research, Technology and Higher Education of the Republic of Indonesia. Publisher Copyright: {\textcopyright} 2021. All Rights Reserved.",

year = "2021",

doi = "10.18585/INABJ.V13I4.1674",

language = "English",

volume = "13",

pages = "355--363",

journal = "Indonesian Biomedical Journal",

issn = "2085-3297",

publisher = "Prodia Education and Research Institute",

number = "4",

}

TY - JOUR

T1 - Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells

AU - Nadia, Yalista Fatia

AU - Syahrani, Resda Akhra

AU - Arumsari, Sekar

AU - Sadikin, Mohamad

AU - Wanandi, Septelia Inawati

N1 - Funding Information: The authors are thankful for the Research Grant (Penelitian Dasar Unggulan Perguruan Tinggi/PDUPT/ PDUPT NKB-2777/UN2.RST/HKP.05.00/2020) from Directorate General of Research and Development Strengthening, Ministry of Research, Technology and Higher Education of the Republic of Indonesia. Publisher Copyright: © 2021. All Rights Reserved.

PY - 2021

Y1 - 2021

N2 - BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES.

AB - BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES.

KW - breast cancer

KW - cancer stem cell

KW - OCT4

KW - proliferation

KW - stemness

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