Effect of bisphenol A and Cu(I) exposure that induces oxidative stress through 8-OHdG formation in physiological conditions

N. F. Suhendra, S. Handayani, Budiawan, I. C. Dani

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This in vitro study was conducted to analyze the formation of biomarker for DNA damage 8-hydroxy-2'-deoxyguanosine (8-OHdG) due to Bisphenol A and metal ion Cu (I) exposure in the presence of H2O2 as oxidizing agent on 2-deoxiguanosine via Fenton-Like reaction in non-physiological conditions (temperature 60°C and pH 8.4). Samples were incubated in numerous incubation time (7 hours and 12 hours) and further analyzed by using UHPLC reverse phase with mobile phase sodium phosphate buffer and methanol (85:15) with UV-Vis detector at wavelength 254 nm. The results showed that 8-OHdG can be formed by reacting Bisphenol A, Cu(I) and H2O2. Mostly, samples with longer incubation time (12 hours) have higher 8-OHdG concentration than shorter incubation time (7 hours). 8-OHdG concentrations in all samples were above LOD value and mostly were above LOQ value. Only one mixture that have 8-OHdG levels below LOQ value, i.e. dG, Cu(I), and H2O2. The highest 8-OHdG concentrations found on the complete mixture of reagents (dG, Bisphenol A, Cu(I), and H2O2) with longer incubation time (12 hours) with concentration equal to 92.438 ppb.

Original languageEnglish
Title of host publication8th International Conference of the Indonesian Chemical Society, ICICS 2019
EditorsMohamad Rafi, Rudi Heryanto, Wulan Tri Wahyuni
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420021
DOIs
Publication statusPublished - 4 Jun 2020
Event8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019 - Bogor, Indonesia
Duration: 6 Aug 20197 Aug 2019

Publication series

NameAIP Conference Proceedings
Volume2243
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019
CountryIndonesia
CityBogor
Period6/08/197/08/19

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