Objective: Gram-negative bacteria in the oral cavity such as Porphyromonas gingivalis and Treponema denticola can adhere on the surface area of the tooth and also in the subgingival area as a biofilm. In this research, pure coconut oil 12.5% mouthwash is used with fermentation method, while based on earlier research using a concentration above 12.5% showed that an antibacterial effect from pure coconut oil will decrease the number of microorganism. Aim: The aim of this study was to analyze the clinical effect microbiological of pure coconut oil 12.5% on the decrease in a number of bacteria P. gingivalis and T. denticola on the margin of porcelain fused to metal crown. Methods: 23 subjects, were patients with porcelain fused to metal crown in posterior of Faculty of Dentisty Universitas Indonesia ‘dental hospital. They fill the informed consent, clinical periodontal examination. Patients were gargled twice daily with a pure coconut oil 12.5% of 30cc for 1 min performed for 4 days. Sampling of saliva were collected with paper point for calculating the number of bacteria P. gingivalis and T. denticola using real-time polymerase chain reaction. Conclusion: Using Pure coconut oil 12.5% showed decrease amount of bacteria P. gingivalis and T. denticola in the margin porcelain fused to metal crown.
- Bacterial colonization
- Porphyromonas gingivalis
- Treponema denticola
- Virgin coconut oil (VCO)12.5%