Effect of β-carotene on cell proliferation and differentiation of adipose-derived stem cells into endothelial progenitor cells

Wahyu Widowati, Caroline Tan Sardjono, Laura Wijaya, Dian Ratih Laksmitawati, Jeanne Adiwinata, Ferry Sandra

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Aim: To evaluate the ability of β-carotene to protect oxidation effect of H2O2, and to enhance proliferation and differentiation into AD-MSCs into EPCs. Experimental: AD-MSCs were induced by EGM Bullet Kit medium with or without β-carotene. The proliferation of EPCs was determined by viable cell number counts. Differentiation into EPCs was characterized by the following surface markers: CD 34, CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2). The protective effect of β-carotene was measured base on the level of intracellular reactive oxygen species (ROS) by fluorescence with 2',7'- dichlorofluorescein diacetate (DCF-DA) using flow cytometry. Results: β-Carotene enhanced cell proliferation and differentiation of AD-MSCs into EPCs, and also decreased the accumulation of H202-induced intracellular ROS in EPCs. Conclusion: β- Carotene plays a role in cell proliferation and differentiation of AD-MSCs to EPCs that might be due to decreased intracellular ROS level.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalBioTechnology: An Indian Journal
Volume9
Issue number10
Publication statusPublished - 1 Jan 2014

Keywords

  • Antioxidant
  • Endothelial progenitor cell
  • Endothelium
  • Oxidative stress
  • Reactive oxygen species (ROS)
  • β-carotene

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