Ease fabrication of PCR modular chip for portable DNA detection kit

Yudan Whulanza; Rifky Aditya; Reyhan Arvialido; Muhammad S. Utomo; Boy M. Bachtiar

doi:10.1063/1.4976791

Ease fabrication of PCR modular chip for portable DNA detection kit

Yudan Whulanza, Rifky Aditya, Reyhan Arvialido, Muhammad S. Utomo, Boy M. Bachtiar

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

17 Citations (Scopus)

Abstract

Engineering a lab-on-a-chip (LoC) to perform the DNA polymerase chain reaction (PCR) for malaria detection is the ultimate goal of this study. This paper investigates the ability to fabricate an LoC kit using conventional method to achieve the lowest production cost by using existing fabrication process. It has been known that majority of LoC was made of polydimethylsiloxane (PDMS) which in this study was realized through a contact mold process. CNC milling process was utilized to create channel features in the range of 150-250 μm on the mold. Characterization on the milling process was done to understand the shrinkage/contraction between mold to product, roughness and also angle of contact of PDMS surface. Ultimately, this paper also includes analysis on flow measurement and heat distribution of an assembled LoC PCR kit. The results show that the achieved dimension of microchannel is 227 μm wide with a roughness of 0.01 μm. The flow measurement indicates a deviation with simulation in the range of 10%. A heat distribution through the kit is achieved following the three temperature zones as desired.

Original language	English
Title of host publication	Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016
Editors	Yudan Whulanza, Sugeng Supriadi, Muhamad Sahlan, Basari
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735414853
DOIs	https://doi.org/10.1063/1.4976791
Publication status	Published - 21 Feb 2017
Event	1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016 - Depok City, Indonesia Duration: 31 May 2016 → 1 Jun 2016

Publication series

Name	AIP Conference Proceedings
Volume	1817
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016
Country/Territory	Indonesia
City	Depok City
Period	31/05/16 → 1/06/16

Keywords

PDMS
lab-on-a-chip
malaria detection
microchannel
polymerase chain reaction

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.4976791

Cite this

Whulanza, Y., Aditya, R., Arvialido, R., Utomo, M. S., & Bachtiar, B. M. (2017). Ease fabrication of PCR modular chip for portable DNA detection kit. In Y. Whulanza, S. Supriadi, M. Sahlan, & Basari (Eds.), Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016 Article 040006 (AIP Conference Proceedings; Vol. 1817). American Institute of Physics Inc.. https://doi.org/10.1063/1.4976791

Whulanza, Yudan ; Aditya, Rifky ; Arvialido, Reyhan et al. / Ease fabrication of PCR modular chip for portable DNA detection kit. Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016. editor / Yudan Whulanza ; Sugeng Supriadi ; Muhamad Sahlan ; Basari. American Institute of Physics Inc., 2017. (AIP Conference Proceedings).

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title = "Ease fabrication of PCR modular chip for portable DNA detection kit",

abstract = "Engineering a lab-on-a-chip (LoC) to perform the DNA polymerase chain reaction (PCR) for malaria detection is the ultimate goal of this study. This paper investigates the ability to fabricate an LoC kit using conventional method to achieve the lowest production cost by using existing fabrication process. It has been known that majority of LoC was made of polydimethylsiloxane (PDMS) which in this study was realized through a contact mold process. CNC milling process was utilized to create channel features in the range of 150-250 μm on the mold. Characterization on the milling process was done to understand the shrinkage/contraction between mold to product, roughness and also angle of contact of PDMS surface. Ultimately, this paper also includes analysis on flow measurement and heat distribution of an assembled LoC PCR kit. The results show that the achieved dimension of microchannel is 227 μm wide with a roughness of 0.01 μm. The flow measurement indicates a deviation with simulation in the range of 10%. A heat distribution through the kit is achieved following the three temperature zones as desired.",

keywords = "PDMS, lab-on-a-chip, malaria detection, microchannel, polymerase chain reaction",

author = "Yudan Whulanza and Rifky Aditya and Reyhan Arvialido and Utomo, {Muhammad S.} and Bachtiar, {Boy M.}",

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Whulanza, Y, Aditya, R, Arvialido, R, Utomo, MS & Bachtiar, BM 2017, Ease fabrication of PCR modular chip for portable DNA detection kit. in Y Whulanza, S Supriadi, M Sahlan & Basari (eds), Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016., 040006, AIP Conference Proceedings, vol. 1817, American Institute of Physics Inc., 1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016, Depok City, Indonesia, 31/05/16. https://doi.org/10.1063/1.4976791

Ease fabrication of PCR modular chip for portable DNA detection kit. / Whulanza, Yudan; Aditya, Rifky; Arvialido, Reyhan et al.
Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016. ed. / Yudan Whulanza; Sugeng Supriadi; Muhamad Sahlan; Basari. American Institute of Physics Inc., 2017. 040006 (AIP Conference Proceedings; Vol. 1817).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Ease fabrication of PCR modular chip for portable DNA detection kit

AU - Whulanza, Yudan

AU - Aditya, Rifky

AU - Arvialido, Reyhan

AU - Utomo, Muhammad S.

AU - Bachtiar, Boy M.

PY - 2017/2/21

Y1 - 2017/2/21

N2 - Engineering a lab-on-a-chip (LoC) to perform the DNA polymerase chain reaction (PCR) for malaria detection is the ultimate goal of this study. This paper investigates the ability to fabricate an LoC kit using conventional method to achieve the lowest production cost by using existing fabrication process. It has been known that majority of LoC was made of polydimethylsiloxane (PDMS) which in this study was realized through a contact mold process. CNC milling process was utilized to create channel features in the range of 150-250 μm on the mold. Characterization on the milling process was done to understand the shrinkage/contraction between mold to product, roughness and also angle of contact of PDMS surface. Ultimately, this paper also includes analysis on flow measurement and heat distribution of an assembled LoC PCR kit. The results show that the achieved dimension of microchannel is 227 μm wide with a roughness of 0.01 μm. The flow measurement indicates a deviation with simulation in the range of 10%. A heat distribution through the kit is achieved following the three temperature zones as desired.

AB - Engineering a lab-on-a-chip (LoC) to perform the DNA polymerase chain reaction (PCR) for malaria detection is the ultimate goal of this study. This paper investigates the ability to fabricate an LoC kit using conventional method to achieve the lowest production cost by using existing fabrication process. It has been known that majority of LoC was made of polydimethylsiloxane (PDMS) which in this study was realized through a contact mold process. CNC milling process was utilized to create channel features in the range of 150-250 μm on the mold. Characterization on the milling process was done to understand the shrinkage/contraction between mold to product, roughness and also angle of contact of PDMS surface. Ultimately, this paper also includes analysis on flow measurement and heat distribution of an assembled LoC PCR kit. The results show that the achieved dimension of microchannel is 227 μm wide with a roughness of 0.01 μm. The flow measurement indicates a deviation with simulation in the range of 10%. A heat distribution through the kit is achieved following the three temperature zones as desired.

KW - PDMS

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KW - microchannel

KW - polymerase chain reaction

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M3 - Conference contribution

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BT - Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices

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A2 - Supriadi, Sugeng

A2 - Sahlan, Muhamad

A2 - Basari, null

PB - American Institute of Physics Inc.

T2 - 1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016

Y2 - 31 May 2016 through 1 June 2016

ER -

Whulanza Y, Aditya R, Arvialido R, Utomo MS, Bachtiar BM. Ease fabrication of PCR modular chip for portable DNA detection kit. In Whulanza Y, Supriadi S, Sahlan M, Basari, editors, Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016. American Institute of Physics Inc. 2017. 040006. (AIP Conference Proceedings). doi: 10.1063/1.4976791

Ease fabrication of PCR modular chip for portable DNA detection kit

Abstract

Publication series

Conference

Keywords

UN SDGs

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