Ease fabrication of PCR modular chip for portable DNA detection kit

Yudan Whulanza, Rifky Aditya, Reyhan Arvialido, Muhammad S. Utomo, B. M. Bachtiar

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Citations (Scopus)

Abstract

Engineering a lab-on-a-chip (LoC) to perform the DNA polymerase chain reaction (PCR) for malaria detection is the ultimate goal of this study. This paper investigates the ability to fabricate an LoC kit using conventional method to achieve the lowest production cost by using existing fabrication process. It has been known that majority of LoC was made of polydimethylsiloxane (PDMS) which in this study was realized through a contact mold process. CNC milling process was utilized to create channel features in the range of 150-250 μm on the mold. Characterization on the milling process was done to understand the shrinkage/contraction between mold to product, roughness and also angle of contact of PDMS surface. Ultimately, this paper also includes analysis on flow measurement and heat distribution of an assembled LoC PCR kit. The results show that the achieved dimension of microchannel is 227 μm wide with a roughness of 0.01 μm. The flow measurement indicates a deviation with simulation in the range of 10%. A heat distribution through the kit is achieved following the three temperature zones as desired.

Original languageEnglish
Title of host publicationBiomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the 1st International Symposium of Biomedical Engineering, ISBE 2016
EditorsYudan Whulanza, Sugeng Supriadi, Muhamad Sahlan, Basari
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735414853
DOIs
Publication statusPublished - 21 Feb 2017
Event1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016 - Depok City, Indonesia
Duration: 31 May 20161 Jun 2016

Publication series

NameAIP Conference Proceedings
Volume1817
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016
CountryIndonesia
CityDepok City
Period31/05/161/06/16

Keywords

  • PDMS
  • lab-on-a-chip
  • malaria detection
  • microchannel
  • polymerase chain reaction

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