E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study

Muhammad Miftahussurur, Kartika Afrida Fauzia, Iswan Abbas Nusi, Poernomo Boedi Setiawan, Ari Fahrial Syam, Langgeng Agung Waskito, Dalla Doohan, Neneng Ratnasari, Ali Khomsan, I. Ketut Adnyana, Junko Akada, Yoshio Yamaoka

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. RESULTS: The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test's results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.

Original languageEnglish
Number of pages1
JournalBMC Research Notes
Volume13
Issue number1
DOIs
Publication statusPublished - 10 Jan 2020

Fingerprint

Helicobacter pylori
Dilution
Agar
Metronidazole
Anti-Bacterial Agents
Levofloxacin
Clarithromycin
Amoxicillin
Testing
Tetracycline
Indonesia
Microbial Drug Resistance

Keywords

  • Agar dilution
  • Antibiotic susceptibility test
  • E-test
  • Helicobacter pylori

Cite this

Miftahussurur, Muhammad ; Fauzia, Kartika Afrida ; Nusi, Iswan Abbas ; Setiawan, Poernomo Boedi ; Syam, Ari Fahrial ; Waskito, Langgeng Agung ; Doohan, Dalla ; Ratnasari, Neneng ; Khomsan, Ali ; Adnyana, I. Ketut ; Akada, Junko ; Yamaoka, Yoshio. / E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori : a comparison study. In: BMC Research Notes. 2020 ; Vol. 13, No. 1.
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Miftahussurur, M, Fauzia, KA, Nusi, IA, Setiawan, PB, Syam, AF, Waskito, LA, Doohan, D, Ratnasari, N, Khomsan, A, Adnyana, IK, Akada, J & Yamaoka, Y 2020, 'E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study', BMC Research Notes, vol. 13, no. 1. https://doi.org/10.1186/s13104-019-4877-9

E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori : a comparison study. / Miftahussurur, Muhammad; Fauzia, Kartika Afrida; Nusi, Iswan Abbas; Setiawan, Poernomo Boedi; Syam, Ari Fahrial; Waskito, Langgeng Agung; Doohan, Dalla; Ratnasari, Neneng; Khomsan, Ali; Adnyana, I. Ketut; Akada, Junko; Yamaoka, Yoshio.

In: BMC Research Notes, Vol. 13, No. 1, 10.01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori

T2 - a comparison study

AU - Miftahussurur, Muhammad

AU - Fauzia, Kartika Afrida

AU - Nusi, Iswan Abbas

AU - Setiawan, Poernomo Boedi

AU - Syam, Ari Fahrial

AU - Waskito, Langgeng Agung

AU - Doohan, Dalla

AU - Ratnasari, Neneng

AU - Khomsan, Ali

AU - Adnyana, I. Ketut

AU - Akada, Junko

AU - Yamaoka, Yoshio

PY - 2020/1/10

Y1 - 2020/1/10

N2 - OBJECTIVE: For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. RESULTS: The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test's results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.

AB - OBJECTIVE: For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. RESULTS: The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test's results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.

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KW - E-test

KW - Helicobacter pylori

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U2 - 10.1186/s13104-019-4877-9

DO - 10.1186/s13104-019-4877-9

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