TY - JOUR
T1 - E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori
T2 - a comparison study
AU - Miftahussurur, Muhammad
AU - Fauzia, Kartika Afrida
AU - Nusi, Iswan Abbas
AU - Setiawan, Poernomo Boedi
AU - Syam, Ari Fahrial
AU - Waskito, Langgeng Agung
AU - Doohan, Dalla
AU - Ratnasari, Neneng
AU - Khomsan, Ali
AU - Adnyana, I. Ketut
AU - Akada, Junko
AU - Yamaoka, Yoshio
N1 - Publisher Copyright:
© 2020 The Author(s).
PY - 2020/1/10
Y1 - 2020/1/10
N2 - OBJECTIVE: For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. RESULTS: The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test's results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.
AB - OBJECTIVE: For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. RESULTS: The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test's results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.
KW - Agar dilution
KW - Antibiotic susceptibility test
KW - E-test
KW - Helicobacter pylori
UR - http://www.scopus.com/inward/record.url?scp=85077765456&partnerID=8YFLogxK
U2 - 10.1186/s13104-019-4877-9
DO - 10.1186/s13104-019-4877-9
M3 - Article
C2 - 31924273
AN - SCOPUS:85077765456
SN - 1756-0500
VL - 13
JO - BMC Research Notes
JF - BMC Research Notes
IS - 1
M1 - 22
ER -