TY - JOUR
T1 - Dosage-related prebiotic effects of inulin in formula-fed infants
AU - Oswari, Hanifah
AU - Widodo, Ariani Dewi
AU - Handayani, Frieda
AU - Juffrie, Mohammad
AU - Sundjaya, Tonny
AU - Bindels, Jacques
AU - Hegar, Badriul
N1 - Funding Information:
and reporting of this study was supported by Prodia, the CRO in a highly professional way. We also acknowledge Dr. Sutantik Endang Wasih Kasunjatan, M.Epid. for excellent statistical analyses and supervision. This study was sponsored by PT Sari Husada Generasi Mahardhika, but the sponsorship had no influence on the outcomes of the study. TS and JB are employees of Danone-Nutricia ELN R&D. None of the authors have any personal or financial conflict of interest to declare.
Publisher Copyright:
© 2019 by The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Purpose: The aim of this study was to identify the minimally meaningful dosage of inulin leading to a prebiotic effect in Indonesian infants. Methods: In a randomized controlled double-blinded, parallel, 3-arm intervention study, 164 healthy formula-fed infants aged 3 to 5 months first obtained formula-A (without inulin) during a 4-week adaptation period. Subsequently, 142 subjects were subjected to a 4-week feeding period by administering either formula-A (no inulin), formula-B (0.2 g/100 mL inulin) or formula-C (0.4 g/100 mL inulin). The primary outcome parameter was %-bifidobacteria in faecal samples determined using quantitative polymerase chain reaction analyses. Secondary outcome parameters were faecal %-lactobacilli, pH and stool frequency, and consistency. Growth and tolerance/adverse effects were recorded as safety parameters. Results: Typical %-bifidobacteria and %-lactobacilli at the end of the adaptation period in the study population were 14% and 2%, respectively. For faecal pH, significant differences between formula groups A vs. C and A vs. B were found at the end of the intervention period. Testing for differences in faecal %-bifidobacteria and %-lactobacilli between groups was hampered by non-normal data set distributions; no statistically significant differences were obtained. Comparisons within groups revealed that only in formula group C, all the three relevant parameters exhibited a significant effect with an increase in faecal %-bifidobacteria and %-lactobacilli and a decrease in pH. Conclusion: A consistent prebiotic effect along with a decrease in pH and increase in %-bifidobacteria and %-lactobacilli was found only in the group administered 0.4 g inulin/100 mL.
AB - Purpose: The aim of this study was to identify the minimally meaningful dosage of inulin leading to a prebiotic effect in Indonesian infants. Methods: In a randomized controlled double-blinded, parallel, 3-arm intervention study, 164 healthy formula-fed infants aged 3 to 5 months first obtained formula-A (without inulin) during a 4-week adaptation period. Subsequently, 142 subjects were subjected to a 4-week feeding period by administering either formula-A (no inulin), formula-B (0.2 g/100 mL inulin) or formula-C (0.4 g/100 mL inulin). The primary outcome parameter was %-bifidobacteria in faecal samples determined using quantitative polymerase chain reaction analyses. Secondary outcome parameters were faecal %-lactobacilli, pH and stool frequency, and consistency. Growth and tolerance/adverse effects were recorded as safety parameters. Results: Typical %-bifidobacteria and %-lactobacilli at the end of the adaptation period in the study population were 14% and 2%, respectively. For faecal pH, significant differences between formula groups A vs. C and A vs. B were found at the end of the intervention period. Testing for differences in faecal %-bifidobacteria and %-lactobacilli between groups was hampered by non-normal data set distributions; no statistically significant differences were obtained. Comparisons within groups revealed that only in formula group C, all the three relevant parameters exhibited a significant effect with an increase in faecal %-bifidobacteria and %-lactobacilli and a decrease in pH. Conclusion: A consistent prebiotic effect along with a decrease in pH and increase in %-bifidobacteria and %-lactobacilli was found only in the group administered 0.4 g inulin/100 mL.
KW - Gastrointestinal microbiome
KW - Infant formula
KW - Inulin
KW - Prebiotics
UR - http://www.scopus.com/inward/record.url?scp=85060277103&partnerID=8YFLogxK
U2 - 10.5223/pghn.2019.22.1.63
DO - 10.5223/pghn.2019.22.1.63
M3 - Article
AN - SCOPUS:85060277103
VL - 22
SP - 63
EP - 71
JO - Pediatric Gastroenterology, Hepatology and Nutrition
JF - Pediatric Gastroenterology, Hepatology and Nutrition
SN - 2234-8646
IS - 1
ER -