Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

A. Ito; M. I. Putra; R. Subahar; M. O. Sato; M. Okamoto; Y. Sako; M. Nakao; H. Yamasaki; K. Nakaya; P. S. Craig; S. S. Margono

doi:10.1079/JOH2002128

Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

A. Ito, M. I. Putra, R. Subahar, M. O. Sato, M. Okamoto, Y. Sako, M. Nakao, H. Yamasaki, K. Nakaya, P. S. Craig, S. S. Margono

Department of Parasitology Pre Clinic

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.

Original language	English
Pages (from-to)	311-314
Number of pages	4
Journal	Journal of Helminthology
Volume	76
Issue number	4
DOIs	https://doi.org/10.1079/JOH2002128
Publication status	Published - Dec 2002

Access to Document

10.1079/JOH2002128

Fingerprint

Dive into the research topics of 'Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis'. Together they form a unique fingerprint.

Cite this

Ito, A., Putra, M. I., Subahar, R., Sato, M. O., Okamoto, M., Sako, Y., Nakao, M., Yamasaki, H., Nakaya, K., Craig, P. S., & Margono, S. S. (2002). Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis. Journal of Helminthology, 76(4), 311-314. https://doi.org/10.1079/JOH2002128

@article{3c89167cee2e4bb8b0d830fbc284cd59,

title = "Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis",

abstract = "Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.",

author = "A. Ito and Putra, {M. I.} and R. Subahar and Sato, {M. O.} and M. Okamoto and Y. Sako and M. Nakao and H. Yamasaki and K. Nakaya and Craig, {P. S.} and Margono, {S. S.}",

note = "Funding Information: This study was supported in part by grants-in-aid for Scientific Research (A) and (B) (10480235, 11694259, 12557024, 14256001) from the Japan Society of Promotion of Science to A. Ito and by Japan Health Science Foundation to S.S. Margono.",

year = "2002",

month = dec,

doi = "10.1079/JOH2002128",

language = "English",

volume = "76",

pages = "311--314",

journal = "Journal of Helminthology",

issn = "0022-149X",

publisher = "Cambridge University Press",

number = "4",

}

Ito, A, Putra, MI, Subahar, R, Sato, MO, Okamoto, M, Sako, Y, Nakao, M, Yamasaki, H, Nakaya, K, Craig, PS & Margono, SS 2002, 'Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis', Journal of Helminthology, vol. 76, no. 4, pp. 311-314. https://doi.org/10.1079/JOH2002128

Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis. / Ito, A.; Putra, M. I.; Subahar, R. et al.
In: Journal of Helminthology, Vol. 76, No. 4, 12.2002, p. 311-314.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

AU - Ito, A.

AU - Putra, M. I.

AU - Subahar, R.

AU - Sato, M. O.

AU - Okamoto, M.

AU - Sako, Y.

AU - Nakao, M.

AU - Yamasaki, H.

AU - Nakaya, K.

AU - Craig, P. S.

AU - Margono, S. S.

N1 - Funding Information: This study was supported in part by grants-in-aid for Scientific Research (A) and (B) (10480235, 11694259, 12557024, 14256001) from the Japan Society of Promotion of Science to A. Ito and by Japan Health Science Foundation to S.S. Margono.

PY - 2002/12

Y1 - 2002/12

N2 - Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.

AB - Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.

UR - http://www.scopus.com/inward/record.url?scp=18744365759&partnerID=8YFLogxK

U2 - 10.1079/JOH2002128

DO - 10.1079/JOH2002128

M3 - Article

C2 - 12498635

AN - SCOPUS:18744365759

SN - 0022-149X

VL - 76

SP - 311

EP - 314

JO - Journal of Helminthology

JF - Journal of Helminthology

IS - 4

ER -

Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this