DNA methylation of P2X3 receptor gene encoded pain marker protein in endometriosis

Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological disorder associated with pelvic pain and infertility. Increasing of P2X3 receptors induced sensitization of pain in endometriosis patients. Epigenetic mechanism such as DNA methylation could lead to alteration of gene expression. The aim of this study was to analyse DNA methylation of P2X3 receptor gene promoter in peritoneal endometriotic tissue from 9 patients compared to endometrial tissue from 9 without endometriosis women as control. The DNA from samples was isolated and with sodium bisulfite converted. We used Methyl Specific PCR (MSP) method to amplify the DNA and then running MSP product in gel electrophoresis. The band intensity of samples were measured by ImageJ software. Statistical analysis was significant correlation between pain and endometriosis (p=0.000). DNA methylation of P2X3 receptor gene promoter among peritoneal endometriotic tissue in women endometriosis and endometrial tissue woman without endometriosis were 100% unmethylated and there was no significant differences (p=0.287), although density of band unmethylated peritoneum endometriosis group was higher than control group. This study was suggesting that DNA methylation of P2X3 receptor gene promoter might be a potential biomarker to early diagnostic of endometriosis without invasive procedure in endometriosis patienst especially with pain symptoms.