Distribution of matrix metalloproteinase-9 (Mmp-9) -1562 C/T gene polymorphism in indonesian males with periodontitis

The aim of this research was to compare the distribution of the MMP-9 -1562 C/T (rs3918242) polymorphism in Indonesian males with and without periodontitis. This descriptive study used 100 stored biological samples. The variation in the MMP-9 (-1562 C/T) polymorphism was investigated by the polymerase chain reaction (PCR) -restriction fragment length (RFLP) method with SphI restriction enzyme digestion and electrophoresis of the resulting fragments. A significant difference was found for MMP-9 -1562 C/T genotypes and alleles between subjects with periodontitis and healthy controls (p < 0.05). The T allele was relatively uncommon, as it appeared in 2% of healthy controls, with no TT genotype observed in subjects with or without periodontitis. Nevertheless, the T allele and CT genotype appeared to be significantly associated with the risk of periodontitis. The results suggested that the T allele of the MMP-9 -1562 C/T polymorphism is significantly associated with periodontitis.