TY - JOUR
T1 - Distribution of E-cadherin 1 (CDH1) promoter methylation in patients with orofacial cleft
AU - Tania, S. F.
AU - Kim, K. R.
AU - Suhartono, A. W.
AU - Latief, Benny Sjarief Sjah
AU - Ibrahim, Elza
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Methylation in the promoter region of the E-cadherin 1 gene, CDH1, which plays an important role in cell-to-cell adhesion and craniofacial morphogenesis, potentially results in gene silencing. The present study aimed to assess the methylation status of CDH1 in Indonesian patients with orofacial clefts as well as the potential association between the methylated gene and cleft occurrence. A total of 24 DNA samples from patients with orofacial cleft and 24 control samples from non-cleft individuals were analyzed using methylation-specific PCR after bisulfite treatment to modify the DNA. The CDH1 methylation status of the samples was determined using electrophoresis. In Indonesian patients with orofacial cleft, 5 (20.83%) samples were fully methylated, 19 (79.17%) were partially methylated, and none were fully unmethylated. In contrast, all control samples were partially unmethylated. However, there was no statistically significant difference in the CDH1 methylation status between patients with orofacial cleft and non-cleft individuals (p > 0.05). Methylation of the CDH1 gene promoter was observed in patients with orofacial cleft. However, there was no significant difference in the frequency of CDH1 promoter methylation between patients with orofacial cleft and non-cleft individuals (p > 0.05).
AB - Methylation in the promoter region of the E-cadherin 1 gene, CDH1, which plays an important role in cell-to-cell adhesion and craniofacial morphogenesis, potentially results in gene silencing. The present study aimed to assess the methylation status of CDH1 in Indonesian patients with orofacial clefts as well as the potential association between the methylated gene and cleft occurrence. A total of 24 DNA samples from patients with orofacial cleft and 24 control samples from non-cleft individuals were analyzed using methylation-specific PCR after bisulfite treatment to modify the DNA. The CDH1 methylation status of the samples was determined using electrophoresis. In Indonesian patients with orofacial cleft, 5 (20.83%) samples were fully methylated, 19 (79.17%) were partially methylated, and none were fully unmethylated. In contrast, all control samples were partially unmethylated. However, there was no statistically significant difference in the CDH1 methylation status between patients with orofacial cleft and non-cleft individuals (p > 0.05). Methylation of the CDH1 gene promoter was observed in patients with orofacial cleft. However, there was no significant difference in the frequency of CDH1 promoter methylation between patients with orofacial cleft and non-cleft individuals (p > 0.05).
UR - http://www.scopus.com/inward/record.url?scp=85054538617&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032064
DO - 10.1088/1742-6596/1073/3/032064
M3 - Conference article
AN - SCOPUS:85054538617
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032064
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -