TY - JOUR
T1 - Dissolution profiles of partially purified bromelain from pineapple cores [Ananas comosus (L.) Merr] encapsulated in glutaraldehyde-crosslinked chitosan
AU - Setiasih, Siswati
AU - Prabowo, Hegi Adi
AU - Budianto, Emil
AU - Hudiyono, Sumi
N1 - Publisher Copyright:
© 2018 Siswati Setiasih et al.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - To avoid degradation in the stomach, the proteolytic enzyme bromelain must be encapsulated in glutaraldehydecrosslinked chitosan (CGF) hydrogels, which can maintain the activity of bromelain until it reaches the intestine. In this study, we isolated bromelain by using ammonium sulfate precipitation, dialysis, and anionic exchange chromatography with Diethylaminoethyl (DEAE)-cellulose resin. Bromelain fractions were collected from each purification step and specific activities were sequentially from increased in the crude enzyme, ammonium sulfate, dialysis, and DEAE chromatography fractions (fraction numbers 58-71), which have fractions of 23.90, 122.00, 125.48, and 195.20 U/mg, respectively. Bromelain fractions from the dialysis step were encapsulated in CGF matrixes by using a post-loading method. CGF hydrogels had a crosslinking degree of 84.37% and swelling ratio of 76.60%. The dissolution profiles of CGF-encapsulated bromelain were tested in artificial stomach fluid and intestinal environments, and bromelain encapsulation efficiency following the post-loading method was 96.29%. Interactions between the hydrogel and bromelain were limited to the hydrogen bonds, and the proteolytic activities of bromelain were maintained at 0.17 U/ml in the present artificial intestinal environment.
AB - To avoid degradation in the stomach, the proteolytic enzyme bromelain must be encapsulated in glutaraldehydecrosslinked chitosan (CGF) hydrogels, which can maintain the activity of bromelain until it reaches the intestine. In this study, we isolated bromelain by using ammonium sulfate precipitation, dialysis, and anionic exchange chromatography with Diethylaminoethyl (DEAE)-cellulose resin. Bromelain fractions were collected from each purification step and specific activities were sequentially from increased in the crude enzyme, ammonium sulfate, dialysis, and DEAE chromatography fractions (fraction numbers 58-71), which have fractions of 23.90, 122.00, 125.48, and 195.20 U/mg, respectively. Bromelain fractions from the dialysis step were encapsulated in CGF matrixes by using a post-loading method. CGF hydrogels had a crosslinking degree of 84.37% and swelling ratio of 76.60%. The dissolution profiles of CGF-encapsulated bromelain were tested in artificial stomach fluid and intestinal environments, and bromelain encapsulation efficiency following the post-loading method was 96.29%. Interactions between the hydrogel and bromelain were limited to the hydrogen bonds, and the proteolytic activities of bromelain were maintained at 0.17 U/ml in the present artificial intestinal environment.
KW - Bromelain
KW - Crosslinked chitosan
KW - Dissolution
KW - Post-loading encapsulation
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=85055961053&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2018.81003
DO - 10.7324/JAPS.2018.81003
M3 - Article
AN - SCOPUS:85055961053
SN - 2231-3354
VL - 8
SP - 17
EP - 24
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 10
ER -