@article{f2d2d92bc2af443c930fea13e5beeed2,
title = "Discovery, isolation, heterologous expression and mode-of-action studies of the antibiotic polyketide tatiomicin from Amycolatopsis sp. DEM30355",
abstract = "A genomic and bioactivity informed analysis of the metabolome of the extremophile Amycolatopsis sp. DEM30355 has allowed for the discovery and isolation of the polyketide antibiotic tatiomicin. Identification of the biosynthetic gene cluster was confirmed by heterologous expression in Streptomyces coelicolor M1152. Structural elucidation, including absolute stereochemical assignment, was performed using complementary crystallographic, spectroscopic and computational methods. Tatiomicin shows antibiotic activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Cytological profiling experiments suggest a putative antibiotic mode-of-action, involving membrane depolarisation and chromosomal decondensation of the target bacteria.",
author = "Bernhard Kepplinger and Lina Mardiana and Joseph Cowell and Stephanie Morton-Laing and Yousef Dashti and Corinne Wills and Marrs, {Emma C.L.} and Perry, {John D.} and Joe Gray and Michael Goodfellow and Jeff Errington and Probert, {Michael R.} and William Clegg and Jonathan Bogaerts and Wouter Herrebout and Allenby, {Nick E.E.} and Hall, {Michael J.}",
note = "Funding Information: The authors thank Newcastle University (NCL) for funding and PhD scholarships (S.M-L. & J.C.), EPSRC (EP/G037620/1) for an EngD studentship (B.K.), the Wellcome Trust (B.K.) for an Investigator Grant to J.E. (209500), the Technology Strategy Board (N.E.E.A.) (100953, 131143), the Rosetrees Trust (M.J.H.) (M543), the Research Foundation Flanders (FWO-Vlaanderen) (J.B.) for a pre-doctoral scholarship (1198318N) the Centre for Process Innovation (CPI) for fermentation and processing support, Diamond Light Source for access to beamline I19 (allocations MT6749 and MT11145), I19 beamline staff for assistance with long-wavelength X-ray experiments, Prof. W. McFarlane (NCL) for NMR support, J. Devi (Demuris) for SEM microscopy, C. Willis (NCL) for the construction of B. subtilis strain CRW419, Prof. A. Bull for collecting the soil sample from which Amycolatopsis sp. DEM30355 was isolated, and Bio S&T for the production of the P1 artificial chromosome (PAC) library. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41598-022-18726-3",
language = "English",
volume = "12",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}