Virtual Reality (VR) has received attention since the trend of the Metaverse came after the pandemic era. Several studies look into how Virtual Reality can be used in higher education. because all the research comes from the education field. However, previous research is too narrow in using technology, but not the area, impact, and challenges of Virtual Reality itself. Therefore, the systematic literature review (SLR) from 2016 to 2022 is discussed in this work to research how discipline, impact, and challenges of Virtual Reality in Higher Education. From 702 papers retrieved, and 214 candidates, to determine the applicability of the study question, 62 papers were chosen. The result shows that most discipline areas are still in computer science, followed by education and public health. Most reports said Virtual Reality is still highly impacted in solving the problem, especially in public health and education. However, there are still some pitfalls: the challenge of using The Head Mounted Device makes Virtual Reality-Induced symptoms and effects (VRISEs) such as Cyber sickness, simulator sickness, Motion sickness, dizziness, and physical discomfort. The implication of the SLR will be considered for academic purposes in preparing for the Metaverse era, especially in higher education.