Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier

Taufiq Indra Rukmana; Ryohei Yasukuni; Gabriela Moran; Rachel Méallet-Renault; Gilles Clavier; Tadashi Kunieda; Misato Ohtani; Taku Demura; Yoichiroh Hosokawa

doi:10.35848/1882-0786/abc490

Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier

Taufiq Indra Rukmana, Ryohei Yasukuni, Gabriela Moran, Rachel Méallet-Renault, Gilles Clavier, Tadashi Kunieda, Misato Ohtani, Taku Demura, Yoichiroh Hosokawa

Faculty of Pharmacy

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Diffusion is an important process for molecular transport inside plant cells. Recent advancement in plant physiological study demands verification of the diffusion process at the single cell level. In this work, the real-time intracellular diffusion of nanoparticles in the cytoplasm of single plant cells was realized using photoinjection with femtosecond laser amplifier. The diffusion behavior was analyzed by estimating the diffusion coefficient in cytoplasm. In addition, the effect of particle size to the photoinjection efficiency and diffusion was evaluated. Surprisingly, the intercellular diffusion of nanoparticles between single plant cells was also directly observed.

Original language	English
Article number	117002
Journal	Applied Physics Express
Volume	13
Issue number	11
DOIs	https://doi.org/10.35848/1882-0786/abc490
Publication status	Published - 1 Nov 2020

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title = "Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier",

abstract = "Diffusion is an important process for molecular transport inside plant cells. Recent advancement in plant physiological study demands verification of the diffusion process at the single cell level. In this work, the real-time intracellular diffusion of nanoparticles in the cytoplasm of single plant cells was realized using photoinjection with femtosecond laser amplifier. The diffusion behavior was analyzed by estimating the diffusion coefficient in cytoplasm. In addition, the effect of particle size to the photoinjection efficiency and diffusion was evaluated. Surprisingly, the intercellular diffusion of nanoparticles between single plant cells was also directly observed.",

author = "Rukmana, {Taufiq Indra} and Ryohei Yasukuni and Gabriela Moran and Rachel M{\'e}allet-Renault and Gilles Clavier and Tadashi Kunieda and Misato Ohtani and Taku Demura and Yoichiroh Hosokawa",

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T1 - Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier

AU - Rukmana, Taufiq Indra

AU - Yasukuni, Ryohei

AU - Moran, Gabriela

AU - Méallet-Renault, Rachel

AU - Clavier, Gilles

AU - Kunieda, Tadashi

AU - Ohtani, Misato

AU - Demura, Taku

AU - Hosokawa, Yoichiroh

PY - 2020/11/1

Y1 - 2020/11/1

N2 - Diffusion is an important process for molecular transport inside plant cells. Recent advancement in plant physiological study demands verification of the diffusion process at the single cell level. In this work, the real-time intracellular diffusion of nanoparticles in the cytoplasm of single plant cells was realized using photoinjection with femtosecond laser amplifier. The diffusion behavior was analyzed by estimating the diffusion coefficient in cytoplasm. In addition, the effect of particle size to the photoinjection efficiency and diffusion was evaluated. Surprisingly, the intercellular diffusion of nanoparticles between single plant cells was also directly observed.

AB - Diffusion is an important process for molecular transport inside plant cells. Recent advancement in plant physiological study demands verification of the diffusion process at the single cell level. In this work, the real-time intracellular diffusion of nanoparticles in the cytoplasm of single plant cells was realized using photoinjection with femtosecond laser amplifier. The diffusion behavior was analyzed by estimating the diffusion coefficient in cytoplasm. In addition, the effect of particle size to the photoinjection efficiency and diffusion was evaluated. Surprisingly, the intercellular diffusion of nanoparticles between single plant cells was also directly observed.

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VL - 13

JO - Applied Physics Express

JF - Applied Physics Express

IS - 11

M1 - 117002

ER -

Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier

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