Differences in the effects of 0.05% and 0.1% propolis flavonoids on in vitro biofilm formation by streptococcus mutans from children’s dental plaque

Objective: This study was conducted to analyze the effects obtained with different concentrations (0.5 and 0.1%) of propolis flavonoids on in vitro biofilm formation by clinical Streptococcus mutans strains isolated from children’s dental plaque. Materials and Methods: S. mutans isolated from children’s dental plaque was assayed for biofilm formation in 96-microwell plates using crystal violet. Results: The effects on S. mutans biofilm formation were the same for propolis flavonoids administered at concentrations of 0.05 and 0.1% (p>0.01). Conclusion: A 0.05% propolis flavonoids concentration was deemed as effective as a 0.1% concentration at inhibiting S. mutans biofilm formation.