TY - JOUR
T1 - Difference of regulatory T cells, IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin
T2 - A research article
AU - Murni, Nani Sari
AU - Sadikin, Mohamad
AU - Rahman, Ichramsjah A.
AU - Mansur, Indra Gusti
N1 - Funding Information:
This project was supported by Beasiswa Unggulan Dosen Indonesia-Lembaga Pengelola Dana Pendidikan (BUDI-LPDP) 2016.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/9
Y1 - 2020/9
N2 - Objective: The maternal immune system requires tolerance for conception to occur. It is not only the balance of Th 1/Th2 that plays a role in pregnancy, but also the regulatory T cells (Tregs) that regulate the important role in pregnancy. One cause of failure in pregnancy is due to immunological factors, including antisperm antibodies (ASA). About 10–30% of infertile couples are caused by ASA. Th1 secretes Interferon γ (IFNγ). IFNγ is also an inducer indoleamin 2,3 dioksigenase (IDO). Cooperation between Tregs and IDO will induce tolerance for pregnancy.Th2 secretes most ofinterleukin (IL)10. Increased IL10 and decreased IL6 occur during pregnancy. The aim of this study was to analyse difference of Tregs, IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin. Methods: Samples with high ASA were examined ASA titres using the husband's sperm auto-agglutination test (HSAaT) method.49 samples were analysed. Tregs were evaluated using flowcytometry with the human forkhead box P3 (FoxP3) staining kit of Biotech and Device.Level of IL10, IL6, and IFNγ was determined using an Abcam ELISA kit. Level of IDO was determined using an RnD ELISA kit. The data were analysed using the Mann-whitney tests. Results: There are differences in the Tregs population (p = 0.000<0.05) but there is no difference IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin (p 0.140 > 0.05, p 0.680 > 0.05, p 0.204 > 0.05, and p 0.362 > 0.05). Conclusion: High ASA affects of the Tregs population but has no effect on cytokines IL10, IL6, IFNγ, and IDO.
AB - Objective: The maternal immune system requires tolerance for conception to occur. It is not only the balance of Th 1/Th2 that plays a role in pregnancy, but also the regulatory T cells (Tregs) that regulate the important role in pregnancy. One cause of failure in pregnancy is due to immunological factors, including antisperm antibodies (ASA). About 10–30% of infertile couples are caused by ASA. Th1 secretes Interferon γ (IFNγ). IFNγ is also an inducer indoleamin 2,3 dioksigenase (IDO). Cooperation between Tregs and IDO will induce tolerance for pregnancy.Th2 secretes most ofinterleukin (IL)10. Increased IL10 and decreased IL6 occur during pregnancy. The aim of this study was to analyse difference of Tregs, IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin. Methods: Samples with high ASA were examined ASA titres using the husband's sperm auto-agglutination test (HSAaT) method.49 samples were analysed. Tregs were evaluated using flowcytometry with the human forkhead box P3 (FoxP3) staining kit of Biotech and Device.Level of IL10, IL6, and IFNγ was determined using an Abcam ELISA kit. Level of IDO was determined using an RnD ELISA kit. The data were analysed using the Mann-whitney tests. Results: There are differences in the Tregs population (p = 0.000<0.05) but there is no difference IL10, IL6, IFNγ, and IDO levels in female with high ASA and virgin (p 0.140 > 0.05, p 0.680 > 0.05, p 0.204 > 0.05, and p 0.362 > 0.05). Conclusion: High ASA affects of the Tregs population but has no effect on cytokines IL10, IL6, IFNγ, and IDO.
KW - IDO
KW - IFNγ
KW - IL10
KW - IL6
KW - Regulatory T cell
KW - Sperm agglutinationASA
UR - http://www.scopus.com/inward/record.url?scp=85086478282&partnerID=8YFLogxK
U2 - 10.1016/j.jri.2020.103156
DO - 10.1016/j.jri.2020.103156
M3 - Article
C2 - 32540614
AN - SCOPUS:85086478282
VL - 141
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
SN - 0165-0378
M1 - 103156
ER -