Dietary nano-calcium phosphate and hydrolyzed collagen for the inhibition of osteoporosis in calcium-deficient bones

R. Sapundani, A. L. Juwono, D. S. Soejoko, D. A. Astuti

Research output: Contribution to journalConference articlepeer-review

Abstract

Several studies exhibit that high calcium intake prevents osteoporosis-related bone loss. In this study, the dietary effects of nano-tricalcium phosphate (n-CaP) and hydrolyzed collagen (HC) against bone loss at the peak of the bone growth stage are examined, and the main objectives are to prevent osteoporosis and to induce bone formation. The Sprague Dawley mice (Rattus norvegicus) is observed to be calcium deficient (the average calcium content of serum is ≤ 8%). The mice were randomized into four groups (P1: normal purified diet, P2: HC purified diet, P3: n-CaP purified diet, and P4: n-CaP and HC purified diet). After four weeks, all the groups were euthanized, and their bone formation was analyzed. UV-Vis analysis revealed that the calcium content values were 8.20 (P1), 8.92 (P2), 9.2 (P3), and 10.35 (P4) mg/dL, respectively. The corresponding values obtained by the atomic absorption spectrophotometric analysis are 72.29 (P1), 74.25 (P2), 74.80 (P3), and 76.04 (P4) w/w% respectively. To assess the spinal calcification, the infrared spectra were recorded. The splitting factors were 2.5 (P1), 2.6 (P2), 2.5 (P3), and 2.6 (P4), indicating better absorption of n-CaP for P4. Hence, n-CaP and HC-purified diets are suitable to ensure bone growth.

Original languageEnglish
Article number012017
JournalIOP Conference Series: Materials Science and Engineering
Volume496
Issue number1
DOIs
Publication statusPublished - 22 Feb 2019
Event2nd International Conference on Current Progress in Functional Materials 2017, ISCPFM 2017 - Bali, Indonesia
Duration: 8 Nov 20179 Nov 2017

Keywords

  • hydrolyzed collagen
  • nano-calcium phosphate
  • Osteoporosis

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