Several studies exhibit that high calcium intake prevents osteoporosis-related bone loss. In this study, the dietary effects of nano-tricalcium phosphate (n-CaP) and hydrolyzed collagen (HC) against bone loss at the peak of the bone growth stage are examined, and the main objectives are to prevent osteoporosis and to induce bone formation. The Sprague Dawley mice (Rattus norvegicus) is observed to be calcium deficient (the average calcium content of serum is ≤ 8%). The mice were randomized into four groups (P1: normal purified diet, P2: HC purified diet, P3: n-CaP purified diet, and P4: n-CaP and HC purified diet). After four weeks, all the groups were euthanized, and their bone formation was analyzed. UV-Vis analysis revealed that the calcium content values were 8.20 (P1), 8.92 (P2), 9.2 (P3), and 10.35 (P4) mg/dL, respectively. The corresponding values obtained by the atomic absorption spectrophotometric analysis are 72.29 (P1), 74.25 (P2), 74.80 (P3), and 76.04 (P4) w/w% respectively. To assess the spinal calcification, the infrared spectra were recorded. The splitting factors were 2.5 (P1), 2.6 (P2), 2.5 (P3), and 2.6 (P4), indicating better absorption of n-CaP for P4. Hence, n-CaP and HC-purified diets are suitable to ensure bone growth.
|Journal||IOP Conference Series: Materials Science and Engineering|
|Publication status||Published - 22 Feb 2019|
|Event||2nd International Conference on Current Progress in Functional Materials 2017, ISCPFM 2017 - Bali, Indonesia|
Duration: 8 Nov 2017 → 9 Nov 2017
- hydrolyzed collagen
- nano-calcium phosphate