Development of double-layered alginate-chitosan hydrogels for human stem cell microencapsulation

Retno Wahyu Nurhayati, Rafianto Dwi Cahyo, Kamila Alawiyah, Gita Pratama, Elizabeth Agustina, Radiana Dhewayani Antarianto, Ani Retno Prijanti, Wildan Mubarok, Ahmad Jabir Rahyussalim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Citations (Scopus)

Abstract

Microencapsulation is a promising technology for stem cell therapy and tissue regeneration. Our previous work proposed the application of microencapsulation technology for improving allogeneic hematopoietic stem cell transplantation. In this current work, we developed a feasible method for encapsulating human umbilical cord blood-derived hematopoietic stem cells. The cells were initially entrapped in ionic cross-linked alginate beads based on a conventional method. It was noticed that alginate beads were easily dissolved in phosphate-buffered saline with Ca2+/Mg2+, suggesting a poor stability of alginate beads. Therefore, a double-layered microcapsule was developed by coating the alginate gel with glutaraldehyde cross-linked chitosan. Measurement of relative swelling ratio showed that the double-layered gel could expand 1.49-fold in a rich culture medium. A homogenous cell distribution could be visualized in solid core alginate microcapsule by a DNA staining. Altogether, this study presented a feasible method to fabricate a double-layered alginate-chitosan microcapsule for human stem cells.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/TerritoryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • alginate
  • chitosan
  • hydrogel
  • microencapsulation
  • stem cells

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