@inproceedings{fe9e4992ecf64e1ca79bfbcb4520639a,
title = "Development of double-layered alginate-chitosan hydrogels for human stem cell microencapsulation",
abstract = "Microencapsulation is a promising technology for stem cell therapy and tissue regeneration. Our previous work proposed the application of microencapsulation technology for improving allogeneic hematopoietic stem cell transplantation. In this current work, we developed a feasible method for encapsulating human umbilical cord blood-derived hematopoietic stem cells. The cells were initially entrapped in ionic cross-linked alginate beads based on a conventional method. It was noticed that alginate beads were easily dissolved in phosphate-buffered saline with Ca2+/Mg2+, suggesting a poor stability of alginate beads. Therefore, a double-layered microcapsule was developed by coating the alginate gel with glutaraldehyde cross-linked chitosan. Measurement of relative swelling ratio showed that the double-layered gel could expand 1.49-fold in a rich culture medium. A homogenous cell distribution could be visualized in solid core alginate microcapsule by a DNA staining. Altogether, this study presented a feasible method to fabricate a double-layered alginate-chitosan microcapsule for human stem cells.",
keywords = "alginate, chitosan, hydrogel, microencapsulation, stem cells",
author = "Nurhayati, {Retno Wahyu} and Cahyo, {Rafianto Dwi} and Kamila Alawiyah and Gita Pratama and Elizabeth Agustina and Antarianto, {Radiana Dhewayani} and Prijanti, {Ani Retno} and Wildan Mubarok and Rahyussalim, {Ahmad Jabir}",
note = "Funding Information: 1Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 2Stem Cells and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 3Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Jl Raya Dramaga, Dramaga, Bogor, Indonesia 4Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 5Integrated Service Unit of Stem Cell Medical Technology (UPT TK Sel Punca), Dr. Cipto Mangunkusumo General Hospital (RSCM), Jl. Diponegoro No 71, Salemba, Jakarta Pusat 10430, Indonesia 6Department of Histology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 7Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia Funding Information: This research was funded by Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) Universitas Indonesia grant from the Indonesian Ministry of Research and Technology and Higher Education awarded to Dr. Rahyussalim (Grant No. NKB-1561/UN2.R3.1/HKP.05.00/2019). The authors thank Dr. Cipto Mangunkusumo General Hospital (RSCM) and Elizabeth Agustina, MD for collecting UCBs and Dr. Suwarti from Infectious Diseases and Immunology Research Center, IMERI, Faculty of Medicine, Universitas Indonesia for Fluorescence Microscope Analysis. Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 ; Conference date: 22-07-2019 Through 24-07-2019",
year = "2019",
month = dec,
day = "10",
doi = "10.1063/1.5139324",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Kenny Lischer and Tomy Abuzairi and Rahman, {Siti Fauziyah} and Misri Gozan",
booktitle = "4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices",
address = "United States",
}