Development of Cr(III) analytical method in dairy products by cloud point extraction using graphite furnace atomic absorption spectroscopy

S. A. Hutapea, A. Saefumillah, E. W. Asijati

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cr(III) is one of chromium (Cr) metal ion species which is essential for human body because it has a significant influence on the metabolism of glucose, fat and protein. To specify the concentration of Cr(III), cloud point extraction was used as a method of separation and preconcentration which is low cost, fast and environmentally friendly. In this study, the analytical method development of Cr(III) in dairy products was performed using 1-(2-pyridylazo)-2-naphthol (PAN) ligand and nonionic surfactant Triton X-114 which was detected by Graphite Furnace Atomic Absorption Spectroscopy. Factors affecting the efficiency of extraction like pH of solution, concentration of ligand and surfactant, equilibration temperature and time were optimized to obtain the optimum extraction efficiency. Addition of Na2SO4 was also carried out to improve the extraction efficiency. The analysis of Cr(III) in dairy products which was previously treated with trichloroacetic acid produces precision with 4.1 % RSD and the recovery range of Cr(III) is of 80.0 to 100.8 %. The detection limit in dairy products obtained is 45 ng/g and the quantization limit is 150 ng/g. The enrichment factor after 125 times surfactant-rich phase dilution is of 2.1 and the preconcentration factor obtained is of 310.3. It is proved that the analytical method using cloud point extraction can be applied to specify the Cr(III) concentration in dairy products.

Original languageEnglish
Title of host publicationProceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
EditorsTerry Mart, Djoko Triyono, Tribidasari Anggraningrum Ivandini
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420014
DOIs
Publication statusPublished - 1 Jun 2020
Event5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019 - Depok, Indonesia
Duration: 9 Jul 201910 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2242
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
Country/TerritoryIndonesia
CityDepok
Period9/07/1910/07/19

Keywords

  • 1-(2-pyridylazo)-2-naphtol (PAN)
  • cloud point extraction
  • Cr(III)
  • dairy products

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