Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture

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1 Citation (Scopus)

Abstract

Bioreactors are growing in popularity among network engineers. Bioreactors are generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system for cell culture that is controlled automatically and remotely using multi-chambers that allow inflow of fresh liquid or output for sample collection. This system facilitates gas exchange between the culture medium and ambient gas. The system provides a storage bag for fresh media and a peristaltic pump is used to move the media in a closed loop during perfusion and stirring in the cell culture chamber. The flow rates used is 2 mL/min. The system has a container for direct sample measurement to obtain pH, oxygen, and carbon dioxide parameters. The bioreactor system is also equipped with a mixing vessel which allows the addition of desired nutrients or additives to the system. The results of a computational fluid dynamics (CFD) and shear stress-based flow analysis have been conducted. The simulation results demonstrate that the applied Pivot parameters correspond to an ideal environment to hepatocyte cell viability and growth.

Original languageEnglish
Pages (from-to)1651-1662
Number of pages12
JournalInternational Journal of Technology
Volume13
Issue number8
DOIs
Publication statusPublished - 2022

Keywords

  • Automated system
  • Bioreactor
  • Cell culture
  • Pivot
  • Remotely controlled

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