TY - JOUR
T1 - Development of an Acrylamide Biosensor Using Guanine and Adenine as Biomarkers at Boron-Doped Diamond Electrodes
T2 - Integrated Molecular Docking and Experimental Studies
AU - Anggraini, Listya Eka
AU - Rahmawati, Isnaini
AU - Nasution, Mochammad Arfin Fardiansyah
AU - Jiwanti, Prastika Krisma
AU - Einaga, Yasuaki
AU - Ivandini, Tribidasari Anggraningrum
N1 - Funding Information:
Tribidasari Anggraningrum Ivandini has been a professor at the Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia since 2018. In 2003 she accomplished her doctoral degree at The University of Tokyo, Japan. Between 2003 and 2007 she performed post-doctoral research at the Department of Chemistry, Keio University, Japan with a JSPS fellowship during 2004–2006. Her interest is electrochemistry of carbon and diamond materials for sensors and other electro-catalytic applications.
Publisher Copyright:
© 2023 The Chemical Society of Japan.
PY - 2023/5
Y1 - 2023/5
N2 - An acrylamide biosensor was developed by utilizing purine bases, i.e. guanine and adenine, through computational and electrochemical approaches. The molecular docking simulation proved that interaction of double-stranded DNA with the purine bases has the lowest Gibbs binding free energy compared to other biomolecules with a ΔGbinding of -4.2759 kcal/mol. Meanwhile, cyclic voltammetry of both guanine and adenine in 0.1M phosphate buffer solution at pH 7.4 using a boron-doped diamond electrode showed an irreversible oxidation peak in the potential range of 0 to +1.8V (vs. Ag/AgCl), confirming that the oxidation reaction was irreversible. The current of these peaks decreased linearly with the concentration of acrylamide due to the adduct formation between the purine bases and acrylamide. The formation of acrylamide adducts between acrylamide and purine bases was confirmed by the shift of the peak wavelength of the UV spectrum from 260 to 257 nm. The use of guanine for acrylamide sensing showed a linear calibration curve in the concentration range of 0.20-1.00 μM (R2 = 0.99) with a limit of detection and limit of quantification attained at 0.11 and 0.36 μM, respectively. In the case of adenine, a linear calibration curve was observed in the concentration range of 0.14-1.00 μM (R2 = 0.99) with a limit of detection and limit of quantification of 0.10 and 0.34 μM, respectively. The developed method was successfully performed for the acrylamide determination in coffee samples and was validated by HPLC.
AB - An acrylamide biosensor was developed by utilizing purine bases, i.e. guanine and adenine, through computational and electrochemical approaches. The molecular docking simulation proved that interaction of double-stranded DNA with the purine bases has the lowest Gibbs binding free energy compared to other biomolecules with a ΔGbinding of -4.2759 kcal/mol. Meanwhile, cyclic voltammetry of both guanine and adenine in 0.1M phosphate buffer solution at pH 7.4 using a boron-doped diamond electrode showed an irreversible oxidation peak in the potential range of 0 to +1.8V (vs. Ag/AgCl), confirming that the oxidation reaction was irreversible. The current of these peaks decreased linearly with the concentration of acrylamide due to the adduct formation between the purine bases and acrylamide. The formation of acrylamide adducts between acrylamide and purine bases was confirmed by the shift of the peak wavelength of the UV spectrum from 260 to 257 nm. The use of guanine for acrylamide sensing showed a linear calibration curve in the concentration range of 0.20-1.00 μM (R2 = 0.99) with a limit of detection and limit of quantification attained at 0.11 and 0.36 μM, respectively. In the case of adenine, a linear calibration curve was observed in the concentration range of 0.14-1.00 μM (R2 = 0.99) with a limit of detection and limit of quantification of 0.10 and 0.34 μM, respectively. The developed method was successfully performed for the acrylamide determination in coffee samples and was validated by HPLC.
KW - Acrylamide biosensor
KW - Boron-doped diamond
KW - Purine bases
UR - http://www.scopus.com/inward/record.url?scp=85160725760&partnerID=8YFLogxK
U2 - 10.1246/bcsj.20230030
DO - 10.1246/bcsj.20230030
M3 - Article
AN - SCOPUS:85160725760
SN - 0009-2673
VL - 96
SP - 420
EP - 428
JO - Bulletin of the Chemical Society of Japan
JF - Bulletin of the Chemical Society of Japan
IS - 5
ER -