TY - JOUR
T1 - Development of a Simple and Rapid HPLC-UV Method for Ultrasound-assisted Deep Eutectic Solvent Extraction optimization of Ferulic Acid and Antioxidant Activity from Ixora javanica Flowers
AU - Oktaviyanti, Nina Dewi
AU - Setiawan, Finna
AU - Kartini, Kartini
AU - Azminah, Azminah
AU - Avanti, Christina
AU - Hayun, Hayun
AU - Mun'im, Abdul
N1 - Funding Information:
We thank for the the financial support provided by The Ministry of Research, Technology and Higher Education of the Republic of Indonesia for PDUPT 2021 grant ref no. NKB-056/UN2.RST/HKP.05.00/2021. Nina Dewi Oktaviyanti was supported by the Universitas Surabaya, Indonesia, with a PhD programme scholarship.
Publisher Copyright:
© 2022
PY - 2022/4
Y1 - 2022/4
N2 - In this work, we developed an environmental friendly extraction method to enhance ferulic acid yields and antioxidant activity of I. javanica flowers using Ultrasound-assisted extraction (UAE) method and Deep Eutectic Solvents as green solvent. To optimize the extraction method, response surface method analysis using Box Behnken designs were conducted. The results showed that the optimum extraction conditions for ferulic acid yields, DPPH scavenging capacity, ABTS scavenging capacity and Ferric-Reducing Antioxidant Power featured an extraction time of 40 min, 25% water content, and solid-to-liquid ratio of 1/27 g/mL. In this study, a rapid and simple analytical method was also validated for the quantification of ferulic acid in I. javanica flower extract using LC-UV method. The developed method was carried out under following condition: isocratic mobile phase of acetonitrile/formic acid pH 2.55 (30:70), a flow rate of 0.8 ml/min, detection at 321 nm, and C-18 column (250 mm x 4.6 mm, 5 µm). The ferulic acid and antioxidant activity of the extract obtained from the developed were higher than ethanolic extract under same extraction condition. In summary, the results of this study can provide more effective and efficient extraction method by applying the green extraction principle and be recommended to provide new potential antioxidant raw material.
AB - In this work, we developed an environmental friendly extraction method to enhance ferulic acid yields and antioxidant activity of I. javanica flowers using Ultrasound-assisted extraction (UAE) method and Deep Eutectic Solvents as green solvent. To optimize the extraction method, response surface method analysis using Box Behnken designs were conducted. The results showed that the optimum extraction conditions for ferulic acid yields, DPPH scavenging capacity, ABTS scavenging capacity and Ferric-Reducing Antioxidant Power featured an extraction time of 40 min, 25% water content, and solid-to-liquid ratio of 1/27 g/mL. In this study, a rapid and simple analytical method was also validated for the quantification of ferulic acid in I. javanica flower extract using LC-UV method. The developed method was carried out under following condition: isocratic mobile phase of acetonitrile/formic acid pH 2.55 (30:70), a flow rate of 0.8 ml/min, detection at 321 nm, and C-18 column (250 mm x 4.6 mm, 5 µm). The ferulic acid and antioxidant activity of the extract obtained from the developed were higher than ethanolic extract under same extraction condition. In summary, the results of this study can provide more effective and efficient extraction method by applying the green extraction principle and be recommended to provide new potential antioxidant raw material.
KW - Ferulic acid, Antioxidant
KW - Ixora javanica, Ultrasound-assisted extraction, Deep eutectic solvents, Response surface methodology
UR - http://www.scopus.com/inward/record.url?scp=85126586425&partnerID=8YFLogxK
U2 - 10.1016/j.sajce.2022.03.004
DO - 10.1016/j.sajce.2022.03.004
M3 - Article
AN - SCOPUS:85126586425
SN - 1026-9185
VL - 40
SP - 165
EP - 175
JO - South African Journal of Chemical Engineering
JF - South African Journal of Chemical Engineering
ER -