The present study aimed to assess whether a newly constructed, catalase-deficient Escherichia coli strain that express mammalian catalase gene could be used to identify oxidative stress-generating chemicals. We tested L-3,4-dihydroxyphenylalanine (L-DOPA), a well-known agent that induces reactive oxygen species. We found that L-DOPA exposure reduced the survival of catalase-mutant E. coli in a dose-dependent manner, especially in the strains with lower catalase activities, implying the usefulness of these strains in assessment of oxidative chemicals.
- Catalase-mutant Escherichia coli
- Hazard assessment
- L-3,4-dihydroxyphenylalanine cytotoxicity
- Reactive oxygen species