Development of a new assay for evaluation of L-3,4-dihydroxyphenylalanine cytotoxicity in catalase-mutant Escherichia coli

D. Hong Wang, Masako Horita, Ken Tsutsui, Kuniaki Sano, Rani Sauriasari, Noriyoshi Masuoka, Yoko Takemura, Tomoko Takigawa, Jiro Takaki, Keiki Ogino

Research output: Contribution to journalArticle

Abstract

The present study aimed to assess whether a newly constructed, catalase-deficient Escherichia coli strain that express mammalian catalase gene could be used to identify oxidative stress-generating chemicals. We tested L-3,4-dihydroxyphenylalanine (L-DOPA), a well-known agent that induces reactive oxygen species. We found that L-DOPA exposure reduced the survival of catalase-mutant E. coli in a dose-dependent manner, especially in the strains with lower catalase activities, implying the usefulness of these strains in assessment of oxidative chemicals.

Original languageEnglish
Pages (from-to)1768-1772
Number of pages5
JournalEnvironmental Toxicology and Chemistry
Volume27
Issue number8
DOIs
Publication statusPublished - 1 Aug 2008

Keywords

  • Catalase-mutant Escherichia coli
  • Hazard assessment
  • L-3,4-dihydroxyphenylalanine cytotoxicity
  • Reactive oxygen species

Fingerprint Dive into the research topics of 'Development of a new assay for evaluation of L-3,4-dihydroxyphenylalanine cytotoxicity in catalase-mutant Escherichia coli'. Together they form a unique fingerprint.

Cite this