TY - JOUR
T1 - Development of a multiassay algorithm (MAA) to identify recent HIV infection in newly diagnosed individuals in Indonesia
AU - Wulan, Wahyu Nawang
AU - Yunihastuti, Evy
AU - Arlinda, Dona
AU - Merati, Tuti Parwati
AU - Wisaksana, Rudi
AU - Lokida, Dewi
AU - Grossman, Zehava
AU - Huik, Kristi
AU - Lau, Chuen Yen
AU - Susanto, Nugroho Harry
AU - Kosasih, Herman
AU - Aman, Abu Tholib
AU - Ang, Sunarto
AU - Evalina, Rita
AU - Ayu Yuli Gayatri, Anak Agung
AU - Hayuningsih, Chakrawati
AU - Indrati, Agnes Rengga
AU - Kumalawati, July
AU - Mutiawati, Vivi Keumala
AU - Realino Nara, Mario Bernardinus
AU - Nurulita, Asvin
AU - Rahmawati, Rahmawati
AU - Rusli, Adria
AU - Rusli, Musofa
AU - Sari, Dewi Yennita
AU - Sembiring, Justina
AU - Udji Sofro, Muchlis Achsan
AU - Susanti, Wiwi Endang
AU - Tandraeliene, Janice
AU - Tanzil, Fransisca Lianiwati
AU - Neal, Aaron
AU - Karyana, Muhammad
AU - Sudarmono, Pratiwi
AU - Maldarelli, Frank
N1 - Funding Information:
We thank the patients who participated in this study and the site study team. This work has been funded in whole or in part with the Ministry of Health Indonesia and the Intramural Research Program, National Institutes of Health; National Cancer Institute, Center for Cancer Research, National Institutes of Health under Contract No ZIA BC 010819. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government. This work was completed in partial fulfilment for the doctorate degree from Universitas Indonesia (W.N.W) and presented at the 36th Annual Meeting of the Japanese Society for AIDS (18–20 November 2022). We thank the INA – RESPOND's INA – PROACTIVE study and the network reference laboratory personnels for their support. Funding: Ministry of Health Indonesia; the Intramural Research Program, National Institutes of Health; National Cancer Institute, Center for Cancer Research, National Institutes of Health, under Contract No ZIA BC 010819. Conceptualization, W.N.W. E.Y. P.P.S. and F.M.; methodology, W.N.W. K.H. Z.G. and F.M.; software, W.N.W.; validation, D.A. D.L. and H.K.; formal analysis, W.N.W. E.Y. H.K. C.Y. and F.M.; investigation, W.N.W. and F.M.; resources, T.P.M. R.W. A.T.A. S.A. R.E. A.A.A.Y.G. C.H. A.R.I. J.K. V.K.M. M.B.R.N. A.N. R.R. A.R. M.R. D.Y.S. J.S. M.A.U.S. W.E.S. J.T. F.L.T. and A.N.; data curation, N.H.S.; writing—original draft preparation, W.N.W.; writing—review and editing, W.N.W. E.Y. D.A. Z.G. K.H. C-Y.L. H.K. and F.M.; visualization, W.N.W. and F.M.; supervision, P.P.S.; project administration, D.A.; funding acquisition, M.K. and F.M. All authors have read and agreed to the published version of the manuscript. The authors declare no conflict of interest. We support inclusive, diverse, and equitable conduct of research. We avoided “helicopter science” practices by including the participating local contributors from the region where we conducted the research as authors on the paper.
Funding Information:
Funding: Ministry of Health Indonesia ; the Intramural Research Program , National Institutes of Health ; National Cancer Institute , Center for Cancer Research , National Institutes of Health , under Contract No ZIA BC 010819 .
Funding Information:
We thank the patients who participated in this study and the site study team. This work has been funded in whole or in part with the Ministry of Health Indonesia and the Intramural Research Program , National Institutes of Health ; National Cancer Institute , Center for Cancer Research , National Institutes of Health under Contract No ZIA BC 010819 . The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government. This work was completed in partial fulfilment for the doctorate degree from Universitas Indonesia (W.N.W) and presented at the 36th Annual Meeting of the Japanese Society for AIDS (18–20 November 2022). We thank the INA – RESPOND’s INA – PROACTIVE study and the network reference laboratory personnels for their support.
Publisher Copyright:
© 2023
PY - 2023/10/20
Y1 - 2023/10/20
N2 - Ongoing HIV transmission is a public health priority in Indonesia. We developed a new multiassay algorithm (MAA) to identify recent HIV infection. The MAA is a sequential decision tree based on multiple biomarkers, starting with CD4+ T cells >200/μL, followed by plasma viral load (pVL) > 1,000 copies/ml, avidity index (AI) < 0 · 7, and pol ambiguity <0 · 47%. Plasma from 140 HIV-infected adults from 19 hospitals across Indonesia (January 2018 – June 2020) was studied, consisting of a training set (N = 60) of longstanding infection (>12-month) and a test set (N = 80) of newly diagnosed (≤1-month) antiretroviral (ARV) drug naive individuals. Ten of eighty (12 · 5%) newly diagnosed individuals were classified as recent infections. Drug resistance mutations (DRMs) against reverse transcriptase inhibitors were identified in two individuals: one infected with HIV subtype C (K219Q, V179T) and the other with CRF01_AE (V179D). Ongoing HIV transmission, including infections with DRMs, is substantial in Indonesia.
AB - Ongoing HIV transmission is a public health priority in Indonesia. We developed a new multiassay algorithm (MAA) to identify recent HIV infection. The MAA is a sequential decision tree based on multiple biomarkers, starting with CD4+ T cells >200/μL, followed by plasma viral load (pVL) > 1,000 copies/ml, avidity index (AI) < 0 · 7, and pol ambiguity <0 · 47%. Plasma from 140 HIV-infected adults from 19 hospitals across Indonesia (January 2018 – June 2020) was studied, consisting of a training set (N = 60) of longstanding infection (>12-month) and a test set (N = 80) of newly diagnosed (≤1-month) antiretroviral (ARV) drug naive individuals. Ten of eighty (12 · 5%) newly diagnosed individuals were classified as recent infections. Drug resistance mutations (DRMs) against reverse transcriptase inhibitors were identified in two individuals: one infected with HIV subtype C (K219Q, V179T) and the other with CRF01_AE (V179D). Ongoing HIV transmission, including infections with DRMs, is substantial in Indonesia.
KW - Computer science
KW - Health sciences
KW - Medicine
UR - http://www.scopus.com/inward/record.url?scp=85173188908&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2023.107986
DO - 10.1016/j.isci.2023.107986
M3 - Article
AN - SCOPUS:85173188908
SN - 2589-0042
VL - 26
JO - iScience
JF - iScience
IS - 10
M1 - 107986
ER -
