Development and validation of methamphetamine analysis in saliva using gas chromatography-tandem mass spectrometry

Yahdiana Harahap, Muhammad Rezqi Hakim, Kuswardani Soedigdo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: The aim of this study was to develop and validate analytical methods for determining methamphetamine in saliva using gas chromatographytandem mass spectrometry (MS). Methods: The chromatography conditions were DB MS-5 capillary columns with a length of 30 m, inner diameter of 0.25 mm, mobile phase of Helium gas 99.999%, flow rate of 0.8 mL/min, detection of MS at m/z values of 58.00 and 91.00, respectively, and ephedrine HCl as the internal standard. Results: The validation of analytical methods for methamphetamine satisfies the validation criteria by the EMEA Guidelines 2011. Bioanalytical methods obtained were linear in the concentration range from 15.0 to 300.0 ng/mL with r>0.9999. Sample preparation was done using liquid-liquid microextraction with cyclohexane, supernatant residue was dried and reconstituted with approximately 100 µL of methanol. Conclusion: The method was successfully applied to saliva samples of methamphetamine users with levels in the range of test.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalInternational Journal of Applied Pharmaceutics
Volume10
Issue numberSpecial Issue 1
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Ephedrine HCl
  • Human saliva
  • Methamphetamine
  • Optimum
  • Validation

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