TY - GEN
T1 - Developing vitamin B12 deficient rat model based on duration of restriction diet
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
AU - Sianipar, Imelda Rosalyn
AU - Ujianti, Irena
AU - Yolanda, Sophie
AU - Murthi, Aditya K.
AU - Amani, Patwa
AU - Santoso, Dewi Irawati Soeria
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 - Many studies indicate a crucial role of vitamin B12 in hyperhomocysteinemia and hyperglicemia. In this study, through dietary restriction, we developed rat models of vitamin B12 deficiencies to elucidate the impact on hyperhomocysteinemia and hyperglycemia. We investigated whether there is a correlation between plasma Vitamin B12 with homocysteine and the presence of hyperglycemia in male rats. Adult male mice were divided into 3 groups (a) Control diet for 16 weeks (b) vitamin B12-restricted diet with pectin as dietary fiber for 8 weeks (K1) (c) vitamin B12-restricted diet with pectin as dietary fiber for 16 weeks (K2). After 8 weeks of feeding, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Blood samples for vitamin B12, homocysteine and glucose were obtained after overnight fasting. After 16 weeks of continued feeding on their respective diets, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Plasma vitamin B12 levels were lower in K2 compared to both the control and K1 groups. On the other hand, the deficient B12 group had significantly higher levels of homocysteine and glucose than control on 8 and 16 weeks. In this study, we concluded that hyperglycemia was associated with lower serum vitamin B12 concentration and serum homocysteine in the development of vitamin B12 deficient rat model.
AB - Many studies indicate a crucial role of vitamin B12 in hyperhomocysteinemia and hyperglicemia. In this study, through dietary restriction, we developed rat models of vitamin B12 deficiencies to elucidate the impact on hyperhomocysteinemia and hyperglycemia. We investigated whether there is a correlation between plasma Vitamin B12 with homocysteine and the presence of hyperglycemia in male rats. Adult male mice were divided into 3 groups (a) Control diet for 16 weeks (b) vitamin B12-restricted diet with pectin as dietary fiber for 8 weeks (K1) (c) vitamin B12-restricted diet with pectin as dietary fiber for 16 weeks (K2). After 8 weeks of feeding, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Blood samples for vitamin B12, homocysteine and glucose were obtained after overnight fasting. After 16 weeks of continued feeding on their respective diets, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Plasma vitamin B12 levels were lower in K2 compared to both the control and K1 groups. On the other hand, the deficient B12 group had significantly higher levels of homocysteine and glucose than control on 8 and 16 weeks. In this study, we concluded that hyperglycemia was associated with lower serum vitamin B12 concentration and serum homocysteine in the development of vitamin B12 deficient rat model.
KW - blood glucose
KW - homocysteine
KW - hyperglycemia
KW - rat model
KW - vitamin B12
UR - http://www.scopus.com/inward/record.url?scp=85064848807&partnerID=8YFLogxK
U2 - 10.1063/1.5096672
DO - 10.1063/1.5096672
M3 - Conference contribution
AN - SCOPUS:85064848807
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
Y2 - 6 August 2018 through 8 August 2018
ER -