Developing vitamin B12 deficient rat model based on duration of restriction diet: Assessment of plasma vitamin B12, homocysteine (Hcy), and blood glucose levels

Imelda Rosalyn Sianipar, Irena Ujianti, Sophie Yolanda, Aditya K. Murthi, Patwa Amani, Dewi Irawati Soeria Santoso

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Many studies indicate a crucial role of vitamin B12 in hyperhomocysteinemia and hyperglicemia. In this study, through dietary restriction, we developed rat models of vitamin B12 deficiencies to elucidate the impact on hyperhomocysteinemia and hyperglycemia. We investigated whether there is a correlation between plasma Vitamin B12 with homocysteine and the presence of hyperglycemia in male rats. Adult male mice were divided into 3 groups (a) Control diet for 16 weeks (b) vitamin B12-restricted diet with pectin as dietary fiber for 8 weeks (K1) (c) vitamin B12-restricted diet with pectin as dietary fiber for 16 weeks (K2). After 8 weeks of feeding, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Blood samples for vitamin B12, homocysteine and glucose were obtained after overnight fasting. After 16 weeks of continued feeding on their respective diets, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Plasma vitamin B12 levels were lower in K2 compared to both the control and K1 groups. On the other hand, the deficient B12 group had significantly higher levels of homocysteine and glucose than control on 8 and 16 weeks. In this study, we concluded that hyperglycemia was associated with lower serum vitamin B12 concentration and serum homocysteine in the development of vitamin B12 deficient rat model.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • blood glucose
  • homocysteine
  • hyperglycemia
  • rat model
  • vitamin B12

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    Sianipar, I. R., Ujianti, I., Yolanda, S., Murthi, A. K., Amani, P., & Santoso, D. I. S. (2019). Developing vitamin B12 deficient rat model based on duration of restriction diet: Assessment of plasma vitamin B12, homocysteine (Hcy), and blood glucose levels. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 [020004] (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096672