Determination of TGFβ1 activity in placental extracts

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Abstract

This study was carried out to investigate the free and total activities of TGFβ1 in placental extracts after boiling for 30 min not heated and heated at 70°C for 30 min. The results showed that: (1) The free TGFβ1 activity before boiling was 10 ± 7-22 ± 9 pg/mg protein, whereas the total TGFβ1 activity was 100 ± 81-240 ± 51 pg/mg protein (steps I and 11 of the extraction procedure). (2) The free TGFβ1 activity after boiling (step III) was not delected, but after acidification to eliminate latency the total TGFβ1 activity in the final placental extract was 165 ±120 pg/mg protein. (3) In the unhealed placenlai preparation, the free TGFβ1 activity was 95 ± 52 pg/mg protein, whereas the total TGFβ1 activity was 838 ± 240 pg/mg protein (step III). In the final placental extract the free TGFβ1 activity was 6 ±2 pg/mg protein, whereas the total TGFβ1 activity was 55 ± 13 pg/mg protein. (4) In the placental preparation heated at 70°C, the free TGFβ1 activity was 12 ±4 pg/mg protein, whereas the total TGFβ1 activity was 379 ± 238 pg/mg protein (step III). In the final placental extract the free TGFβ1 activity was 17±8 pg/mg protein, whereas the total TGFβ1 activity was 242 ±118 mg/protein. These results showed that most of TGFβ1 activity was in its latent form and that in order to obtain a high TGFβ1 activity the extraction procedure should be performed at 7°C or not healed.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalMedical Journal of Indonesia
Volume7
Issue number3
DOIs
Publication statusPublished - 1 Jul 1998

Keywords

  • Activity
  • Placenta
  • TGFβ

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