Determination of specific and non-specific parameters of the simplicia and ethanol extract of sangketan (Achyranthes Aspera L.) leaves

Objective: With recent advancements in traditional medicine, there is an increasing need for quality assurance assessments using standardized processes. In this study, we aimed to determine the specific and non-specific parameters of the Simplicia and 70% ethanol extract of Achyranthes aspera leaves from three different regions. Methods: Samples were extracted using a maceration method with 70% ethanol as the solvent. The tests for the specific and non-specific parameters were based on the Herbal Pharmacopoeia of Indonesia. Results: Our results of the specific parameters of the Simplicia showed that the ethanol-soluble extract contained 5.83-9.36% and the water-soluble extract contained 10.25-15.44%. The chromatogram pattern used p-sitosterol as the standard, and the total phenolic content was 0.93-1.15 mg gallic acid equivalent (GAE)/g Simplicia. The results of the non-specific parameters of Simplicia showed a loss on drying of 15.25-15.91%, a total ash content of 14.58-20.79%, and an acid-insoluble ash value of 1.75-2.19%. Conclusion: Our results of the specific parameters of A. aspera extract showed a total phenolic content of 6.49-7.68% mg GAE/g extract. Our results of the non-specific parameters of the extract showed a total ash value of 13.18-14.52%, an acid-insoluble ash value of 0.14-0.29%, and a moisture content of 10.42-11.16%.