Determination of sibutramine adulterated in herbal slimming products using TLC densitometric method

Hayun, Baitha P. Maggadani, Nurul Amalina

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Determination of sibutramine adulterated in herbal slimming product using thin layer chromatography (TLC) densitometric method with TLC silica gel 60 F 254 aluminium plate as stationary phase and mixture of toluen-diethylamine (10:0.3) as mobile phase has been developed. The calibration curve in the concentration range of 0.50 to 5.00μg/spot showed good linier relationship (r 2 = 0.9986). The limit of detection and quantitation (LOD and LOQ) were 217.5ng and 724.9ng/spot, respectively. The method gave satisfactory specificity, linierity, precision and accuracy validation criteria and was applied for determination of sibutramine in herbal slimming products obtained from several drugstrores in Depok City, West Java, Indonesia. Results of the determination showed that six of seven samples analyzed were detected containing sibutramine HCl with the concentration of 2.45-26.24mg in a single dosage of slimming herbal products.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalIndonesian Journal of Pharmacy
Volume27
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016

Keywords

  • Densitometry
  • Herbal slimming products
  • Sibutramine HCl
  • TLC
  • Validation method

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