Determination of Genotype of Dengue Virus Serotype 1 by Using Primer Design

Cita Christine Mayorita, Beti Ernawati Dewi

Research output: Contribution to journalArticlepeer-review

Abstract

Dengue fever has become a worldwide health problem. This disease occurs more and more frequently and often cause death, especially in some Asian countries including Indonesia. The purpose of this study was to determine the genotype of dengue virus serotype 1 in Indonesia by using primer design as a base to take part in the development of diagnostics and vaccines of the dengue virus. This research consisted of 100 respondents; male and female aged 14-60 years. All samples were selected by consecutive sampling and dengue viruses used in this study were randomly selected in March-December 2010. The next step was sequencing process in January-October 2011 in the Department of Microbiology FKUI by using cross sectional design. The result of this study was dengue virus serotype 1 strains from Indonesia belonged to genotype 4.
Original languageEnglish
JournaleJournal Kedokteran Indonesia
Volume2
Issue number1
DOIs
Publication statusPublished - Apr 2014

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