Determination of formaldehyde content in wet noddles using thin layer chromatography (TLC) densitometric method after derivatization with Nash reagent has been developed. The calibration curve in the concentration range of 4 to 40 ng/spot showed good linier relationship (r = 0.999). The limit of detection and quantitation (LOD and LOQ) were 1.75 and 5.29 ng/spot, respectively. The method gave satisfactory selectivity, linierity, precision and accuracy validation criteria and was applied for determination of formaldehyde content in three wet noodles samples products purchased from traditional market in Depok City, West Java, Indonesia. Results of the determination showed that all samples were detected containing formaldehyde with the concentration of 990.94, 801.80 and 1684.36 mg formaldehyde per kg samples.
- Nash reagent
- Wet noodles