TY - GEN
T1 - Determination of flavonoid content of mixed herbs extract using colorimetric method and thin layer chromatography (TLC)
AU - Tristantini, Dewi
AU - Jessica, Adeline
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 - This paper analyzed Mimusops elengi leaves, Averrhoa carambola leaves and Curcuma xanthorrhiza rhizomes extract. This study aimed to figure out flavonoid contain especially quercetin in the mixed herbs extract. Various particle size and amount of the mixed herbs were tested to determine effect of particle size and amount of substance to flavonoid content of the extract. The extract was analyzed using colorimetric method and thin layer chromatography (TLC). Thus, results of these analyses would depict high flavonoid content especially quercetin. Quercetin was present in the mixed herbs extract through TLC and colorimetric analysis. Through TLC method, flavonoid and quercetin content were measured. Optimum result was obtained by extracting mass of herbs 2.4 grams with particle size of 60 < D <16 mesh powder and dissolving them in 200mL of water. It was equivalent to 498.09ppm of quercetin concentration and total flavonoid content 41.51mg QE/g herbs. Further addition of mass of the mixed herbs caused decrease of flavonoid content due to equilibrium. Meanwhile, fine powder D ≤ 60 mesh had less interaction with solvent during extraction due to floating particles of mixed herbs. It led to less quercetin and total flavonoid content.
AB - This paper analyzed Mimusops elengi leaves, Averrhoa carambola leaves and Curcuma xanthorrhiza rhizomes extract. This study aimed to figure out flavonoid contain especially quercetin in the mixed herbs extract. Various particle size and amount of the mixed herbs were tested to determine effect of particle size and amount of substance to flavonoid content of the extract. The extract was analyzed using colorimetric method and thin layer chromatography (TLC). Thus, results of these analyses would depict high flavonoid content especially quercetin. Quercetin was present in the mixed herbs extract through TLC and colorimetric analysis. Through TLC method, flavonoid and quercetin content were measured. Optimum result was obtained by extracting mass of herbs 2.4 grams with particle size of 60 < D <16 mesh powder and dissolving them in 200mL of water. It was equivalent to 498.09ppm of quercetin concentration and total flavonoid content 41.51mg QE/g herbs. Further addition of mass of the mixed herbs caused decrease of flavonoid content due to equilibrium. Meanwhile, fine powder D ≤ 60 mesh had less interaction with solvent during extraction due to floating particles of mixed herbs. It led to less quercetin and total flavonoid content.
KW - Averrhoa carambola
KW - colorimetric
KW - Curcuma xanthorrhiza
KW - Mimusops elengi
KW - quercetin
KW - thin layer chromatography
UR - http://www.scopus.com/inward/record.url?scp=85064809976&partnerID=8YFLogxK
U2 - 10.1063/1.5096708
DO - 10.1063/1.5096708
M3 - Conference contribution
AN - SCOPUS:85064809976
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Y2 - 6 August 2018 through 8 August 2018
ER -