Determination of flavonoid content of mixed herbs extract using colorimetric method and thin layer chromatography (TLC)

Dewi Tristantini, Adeline Jessica

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper analyzed Mimusops elengi leaves, Averrhoa carambola leaves and Curcuma xanthorrhiza rhizomes extract. This study aimed to figure out flavonoid contain especially quercetin in the mixed herbs extract. Various particle size and amount of the mixed herbs were tested to determine effect of particle size and amount of substance to flavonoid content of the extract. The extract was analyzed using colorimetric method and thin layer chromatography (TLC). Thus, results of these analyses would depict high flavonoid content especially quercetin. Quercetin was present in the mixed herbs extract through TLC and colorimetric analysis. Through TLC method, flavonoid and quercetin content were measured. Optimum result was obtained by extracting mass of herbs 2.4 grams with particle size of 60 < D <16 mesh powder and dissolving them in 200mL of water. It was equivalent to 498.09ppm of quercetin concentration and total flavonoid content 41.51mg QE/g herbs. Further addition of mass of the mixed herbs caused decrease of flavonoid content due to equilibrium. Meanwhile, fine powder D ≤ 60 mesh had less interaction with solvent during extraction due to floating particles of mixed herbs. It led to less quercetin and total flavonoid content.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/TerritoryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • Averrhoa carambola
  • colorimetric
  • Curcuma xanthorrhiza
  • Mimusops elengi
  • quercetin
  • thin layer chromatography

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