The prevalence of stress on various groups in Indonesia is quite high and has not been known their determinants. This study aimed to find out determinants of stress among civil servants at the Health Ministry of Republic of Indonesia. This cross sectional study was conducted in 2013 toward 230 secretariat general civil servants selected randomly. Analysis of statistic used multiple logistic regression. Respondents were considered stress if they got score 28 or more by using 17 questions personal stress inventory. Results showed that prevalence of stress related to and determinants of stress were obesity, age, position, tribe, education and physical activity was worth 79%. The risk of stress was higher among obese civil servants (ORadj = 1.9), age under 40 years old (ORadj = 2.1), tribe Sundanese (ORadj = 3.1), structural positions (ORadj = 2.0), senior high school or vocation level (ORadj = 2.8), women with lack of physical activity (ORadj = 8.2). To sum up, determinants of stress very depended on work loads and individual characteristics, the highest risk of stress among women who lack of physical activity. The Health Ministry should promote health public concerning healthy lifestyle and prevention of stress to all civil servants, periodically holding recreation together, conducting regular exercise on Friday morning in order to reduce obesity and stress.