TY - JOUR
T1 - Detection of Red-Eared Slider (Trachemys scripta elegans) using Environmental DNA with Cytochrome b Primer
AU - Risti, Shafa Talitha
AU - Maryanto, Andi Eko
AU - Andayani, Noviar
N1 - Funding Information:
This study was financially supported by the Faculty of Mathematics and Natural Sciences, Department of Biology, Universitas Indonesia.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2022
Y1 - 2022
N2 - Biomonitoring surveys for the presence of invasive species need effective and efficient methods. Molecular methods based on the detection of genetic material in environmental samples or environmental DNA (eDNA) provide better resolution for detection, especially in elusive species. The eDNA-based method is an approach that has been widely used for conservation and business management because it is a non-invasive method. Cytochrome b primer is a specific primer that can be used to amplify Red-Eared Slider eDNA from water samples, eDNA was amplified using qPCR. The results obtained after performing qPCR are in the form of Ct values that can be used to estimate the biomass of Red-Eared Slider.
AB - Biomonitoring surveys for the presence of invasive species need effective and efficient methods. Molecular methods based on the detection of genetic material in environmental samples or environmental DNA (eDNA) provide better resolution for detection, especially in elusive species. The eDNA-based method is an approach that has been widely used for conservation and business management because it is a non-invasive method. Cytochrome b primer is a specific primer that can be used to amplify Red-Eared Slider eDNA from water samples, eDNA was amplified using qPCR. The results obtained after performing qPCR are in the form of Ct values that can be used to estimate the biomass of Red-Eared Slider.
UR - http://www.scopus.com/inward/record.url?scp=85146586198&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/1111/1/012052
DO - 10.1088/1755-1315/1111/1/012052
M3 - Conference article
AN - SCOPUS:85146586198
SN - 1755-1307
VL - 1111
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012052
T2 - 3rd International Symposium of Earth, Energy, Environmental Science, and Sustainable Development
Y2 - 27 August 2022 through 28 August 2022
ER -