TY - GEN
T1 - Detection of fungi from old Chinese manuscripts in Central Library Universitas Indonesia
AU - Fitri, Reno
AU - Oetari, Ariyanti
AU - Rahmadewi, Madinna
AU - Manullang, Mariata Arisanti
AU - Rachmania, Mazytha Kinanti
AU - Susetyo-Salim, Tamara
AU - Sjamsuridzal, Wellyzar
N1 - Publisher Copyright:
© 2016 Author(s).
PY - 2016/4/19
Y1 - 2016/4/19
N2 - Old manuscripts, as organic materials, can be subjected to deterioration by fungi [1]. Fungal species (over 200) are the main cause of damage to objects of cultural heritage made of or supported on paper [2]. Fungi from old manuscripts can be identified to species level by analysis of Internal Transcribed Spacer (ITS) region of rDNA [3]. Old Chinese manuscripts in Indonesia are one of historical evidences of close relationship between Indonesia and China for hundred years. From our preliminary survey, the collection of old Chinese manuscripts in Central Library Universitas Indonesia was deteriorated by fungi. Information about fungal species from old Chinese manuscripts has not been reported yet, both in Indonesia and China. Our study is aimed to detect the growth of fungi on old Chinese manuscripts, and to isolate and identify the fungi that deteriorates the manuscripts in Central Library Universitas Indonesia. Twelve samples of old Chinese manuscripts deteriorated by fungi shows spore formation and brown spot on their paper surface. Forty one fungal isolates are collected from seven manuscripts, and many of them (28 isolates from 41) are xerophiles. Fifteen isolates were identified by molecular approach using ITS5. They were identified as Anthostomella sp., Aspergillus aculeatus, Aspergillus insuetus, Chaetosartorya stromatoides, Cladosporium colocasiae, Cladosporium columbiae, Cladosporium pini-ponderosa, Flavomyces fulophazii, Fusarium equiseti, Fusarium sp., and Pseudozyma sp. Fifteen fungal isolates could be identified to genus level based on morphological characters as follows: Aspergillus (10 isolates), Curvularia (1 isolate), and Penicillium (4 isolates). Some isolates could not be identified to the genus level since they do not produce asexual/sexual spores (mycelia sterilia). Further identification to species level will be carried out by molecular approach. Most of the isolated fungi from old Chinese manuscripts were xerophiles and able to use Chinese paper as a substrate. This study is the first report on the diversity of fungi from old Chinese manuscripts from Indonesia.
AB - Old manuscripts, as organic materials, can be subjected to deterioration by fungi [1]. Fungal species (over 200) are the main cause of damage to objects of cultural heritage made of or supported on paper [2]. Fungi from old manuscripts can be identified to species level by analysis of Internal Transcribed Spacer (ITS) region of rDNA [3]. Old Chinese manuscripts in Indonesia are one of historical evidences of close relationship between Indonesia and China for hundred years. From our preliminary survey, the collection of old Chinese manuscripts in Central Library Universitas Indonesia was deteriorated by fungi. Information about fungal species from old Chinese manuscripts has not been reported yet, both in Indonesia and China. Our study is aimed to detect the growth of fungi on old Chinese manuscripts, and to isolate and identify the fungi that deteriorates the manuscripts in Central Library Universitas Indonesia. Twelve samples of old Chinese manuscripts deteriorated by fungi shows spore formation and brown spot on their paper surface. Forty one fungal isolates are collected from seven manuscripts, and many of them (28 isolates from 41) are xerophiles. Fifteen isolates were identified by molecular approach using ITS5. They were identified as Anthostomella sp., Aspergillus aculeatus, Aspergillus insuetus, Chaetosartorya stromatoides, Cladosporium colocasiae, Cladosporium columbiae, Cladosporium pini-ponderosa, Flavomyces fulophazii, Fusarium equiseti, Fusarium sp., and Pseudozyma sp. Fifteen fungal isolates could be identified to genus level based on morphological characters as follows: Aspergillus (10 isolates), Curvularia (1 isolate), and Penicillium (4 isolates). Some isolates could not be identified to the genus level since they do not produce asexual/sexual spores (mycelia sterilia). Further identification to species level will be carried out by molecular approach. Most of the isolated fungi from old Chinese manuscripts were xerophiles and able to use Chinese paper as a substrate. This study is the first report on the diversity of fungi from old Chinese manuscripts from Indonesia.
UR - http://www.scopus.com/inward/record.url?scp=84984562165&partnerID=8YFLogxK
U2 - 10.1063/1.4946972
DO - 10.1063/1.4946972
M3 - Conference contribution
AN - SCOPUS:84984562165
T3 - AIP Conference Proceedings
BT - International Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
A2 - Mart, Terry
A2 - Triyono, Djoko
PB - American Institute of Physics Inc.
T2 - 1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
Y2 - 3 November 2015 through 4 November 2015
ER -