Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users

Dian Retno Utari, Budiawan, Elza Ibrahim Auerkari

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors.

Original languageEnglish
JournalSaudi Journal of Biological Sciences
DOIs
Publication statusAccepted/In press - 1 Jan 2020

Keywords

  • 8-OHdG
  • Forensic odontology
  • Forensic toxicology
  • Ni-Cr
  • Nickel

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