Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users

Dian Retno Utari; Elza Ibrahim Auerkari

doi:10.1016/j.sjbs.2020.03.006

Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users

Dian Retno Utari, Budiawan, Elza Ibrahim Auerkari

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors.

Original language	English
Pages (from-to)	1643-1648
Number of pages	6
Journal	Saudi Journal of Biological Sciences
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.sjbs.2020.03.006
Publication status	Published - Jun 2020

Keywords

8-OHdG
Forensic odontology
Forensic toxicology
Ni-Cr
Nickel

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10.1016/j.sjbs.2020.03.006

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title = "Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users",

abstract = "Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors.",

keywords = "8-OHdG, Forensic odontology, Forensic toxicology, Ni-Cr, Nickel",

author = "Utari, {Dian Retno} and Budiawan and Auerkari, {Elza Ibrahim}",

note = "Funding Information: Financial support from Universitas Indonesia to EIA grant number NKB-0249/UN2.R3.1/HKP.05.00/2019 is gratefully appreciated. Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = jun,

doi = "10.1016/j.sjbs.2020.03.006",

language = "English",

volume = "27",

pages = "1643--1648",

journal = "Saudi Journal of Biological Sciences",

issn = "1319-562X",

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AU - Utari, Dian Retno

AU - Budiawan, null

AU - Auerkari, Elza Ibrahim

PY - 2020/6

Y1 - 2020/6

N2 - Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors.

AB - Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors.

KW - 8-OHdG

KW - Forensic odontology

KW - Forensic toxicology

KW - Ni-Cr

KW - Nickel

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Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users

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Keywords

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