Presently, only a handful of research focuses on the development of interface design for e-Learning modules based on various learning styles. The current studies on this field are still limited to some usability issues which ranging from inadequate usability of the proposed design to exclusion of important user research. Nevertheless, interface design is undoubtedly a crucial aspect influencing the success of an e-Learning system implementation. This paper outlines a user-centered study focusing on the development of an alternative interface design of e-Learning modules based on Felder-Silverman learning styles and User Centered Design approach by utilizing mixed methods. The study analyzes users' learning styles, learning object preferences, interface preference, and pain points extracted from a user research which includes semi-structured interviews and certain coding techniques. The results are used as a primary guideline in order to create an alternative interface design for eLearning modules. Three out of four proposed alternative designs have better usability than the designs from the previous study based on System Usability Score measurement.